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Artificial cultivation and production method of lactarius deliciosus

A technology of artificial cultivation and production method, which is applied in the fields of cultivation, plant cultivation, mushroom cultivation, etc., and can solve the problems such as no successful artificial cultivation of chrysalis, unsatisfactory effect, and low output.

Inactive Publication Date: 2020-02-07
CANGZHOU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chrysalis generally parasitizes the base of thatch plants, solitary or in clusters, and it is easy to grow after continuous light rain in summer, but due to the limitation of natural resource conditions such as ambient temperature, humidity, climate, and thatch land conditions, the output is small and unstable, which is difficult to meet People's needs, especially the dry mushroom products have become treasures for relatives and friends
[0003] At present, the chrysalis grows wildly, and the breeding and cultivation of the chrysalis through the artificial breeding method of conventional edible fungus is often not ideal; so far, there is no report on the successful artificial cultivation of the chrysalis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Carry out the artificial cultivation of chrysalis as follows:

[0026] (1) Select a wild chrysostomy strain with typical traits and good growth, and use the tissue separation method to obtain multiple mycelium blocks near the junction of the fruiting body stipe and gills, each grain size is the size of mung bean grains, and use the tissue separation method as The original seed is immediately used for the inoculation operation of step (2);

[0027] (2) Take 200g of dried thatch grass, add 2000g of water to soak for 30min, boil until the water boils and maintain a slight boil for 30min, filter, and the obtained filtrate is the thatch extract; add 200g of potato slices, 20g of glucose, and 20g of agar to the said thatch extract, and boil on high heat Keep boiling for 20 minutes at medium heat, filter with four layers of gauze, and obtain 1000 ml of the filtrate after cooling to be the coriander-PDA composite medium; take an appropriate amount of the coriander-PDA composite...

Embodiment 2

[0032] After the fruiting body described in Example 1, that is, the fruiting body of the chrysalis, continue to control the temperature of the greenhouse at 24-28° C. and the relative humidity of 85-90%, keep proper ventilation and shading, and there will also be the fruiting body of the chrysanthemum from The 4-6cm wide thin soil belt between the end of the soil-covered culture medium continues to grow out, and develops to a mature state that can be harvested; 4 stubbles can be harvested continuously; the average total output of chrysalis per square meter of cultivation bed 1.2kg in total.

[0033] Repeat the preparation steps of the autoclaved bag material in step (3) of Example 1, and prepare 6 bags of sterilized bag material in total for the following use when needed.

Embodiment 3

[0061] While carrying out embodiment 1 step (4)-(5) operation, basically repeat the operation of carrying out these two steps, difference is to use the black plastic film that does not contain herbicide instead in step (4). Gained first stubble chrysalis product, the average yield per square meter of cultivation bed is 0.51kg; After gathering the first stubble, continue to control greenhouse temperature 24-28 ℃, relative humidity 85-90% by the method of embodiment 2, keep With proper ventilation and shading, it is possible to continuously harvest 4 stubbles of scutellaria products; the average total output of scutellaria per square meter of cultivation bed is 1.45kg.

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Abstract

The invention provides an artificial cultivation and production method of lactarius deliciosus, including the steps of (1), selecting strain of wild lactarius deliciosus, separating to obtain the fruiting body of lactarius deliciosus, and taking the fruiting body as the stock culture of lactarius deliciosus; (2), preparing a thatch-PDA composite culture medium, placing the culture medium in a sterile culture vessel, inoculating the stock culture and culturing the mother strain; (3), soaking thatch with water, adding cotton seed hull, wheat bran and lime with blending and mixing uniformly, packaging, sterilizing and cooling, inoculating the mother strain of lactarius deliciosus, culturing to obtain cultivated strain of lactarius deliciosus; (4), blending dry lactarius deliciosus, cotton seed hull, wheat bran and lime with mixing uniformly, adding water, stacking to ferment to obtain a cultivation medium, spreading the cultivation medium to a cultivation bed on the ground of a greenhouse, sowing the cultivated strain of lactarius deliciosus, mulching, controlling conditions of the greenhouse to improve growth of lactarius deliciosus; (5), removing the mulch, covering soil strips, spraying to water the earthing layer completely after full growth of hypha of lactarius deliciosus, further, controlling conditions of the greenhouse, harvesting the fruiting body of lactarius deliciosusafter the fruiting body becomes flat and open, so as to obtain products of lactarius deliciosus.

Description

Background technique [0001] The large area of ​​saline-alkaline wasteland in Bohai Bay is mainly dominated by Imperata praecox, and the associated plants include reeds, bicolor limonium, velvetleaf, radish, zoysia, sinensis limonium, apocynum, foxtail, thistle, tamarisk etc., with a total coverage of about 70%; the Imperata coronata community is the last herbaceous vegetation in the succession from saline meadow vegetation to woody plants. In the saline-alkali thatched land, there is a unique edible fungus - the chrysalis. The local people fight to pick it in the rain during the season when the chrysalis grows. They either eat it fresh or dry it in the sun for storage. [0002] Mycelia is composed of two parts: mycelium and fruiting body. The mycelium is the vegetative organ, which is white and filamentous; The diameter of the cover is about 0.5-2cm. The chrysalis generally parasitizes the base of thatch plants, solitary or in clusters, and it is easy to grow after continuou...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 孔德平王增池阎旭东
Owner CANGZHOU ACAD OF AGRI & FORESTRY SCI
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