Apparatus and method for isolating single particles from a particle suspension
A particle separation and suspension technology, used in the separation of particles, biological cells, cell components or biological macromolecules, processing and recovery of single biological cells or cell subgroups, and cell aggregates to achieve compact structures and improve recovery rates. Effect
Pending Publication Date: 2020-02-07
西尼昂有限公司 +1
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Problems solved by technology
However, there are disadvantages due to the fact that cell handling in the channels of the microfluidic system can alter the cells
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[0032] Features of preferred embodiments of the invention are described below with reference to a particle separation apparatus comprising a droplet distributor arrangement having a single piezoelectric droplet distributor. The present invention is not limited to this embodiment. A series of droplet dispensers can be used, which has the advantage of enabling parallel operation with multiple suspension samples. Furthermore, other types of droplet dispensers may be used instead of piezoelectric droplet dispensers. Furthermore, the practice of the invention is not limited to the specific configuration of the pump and valve arrangements in the figures. In particular, the syringe pump can be replaced by another mechanical pump device, and the bottle-shaped system liquid reservoir of the siphon pump device can be replaced by another type of reservoir.
[0033] Embodiments of the invention are described with reference to the treatment of biological particles, particularly biologica...
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The present invention discloses a particle isolation apparatus 100 for isolating particles from a suspension sample, wherein the particle isolation apparatus 100 comprises a droplet dispenser device 10 for collecting the suspension sample from a carrier substrate 20 and for dispensing droplets onto a target substrate 30, a mechanical pump device 40 being coupled with the droplet dispenser device 10 for loading a dilution liquid into the droplet dispenser device 10 and for aspirating a first portion of the suspension sample into the droplet dispenser device 10, and a syphon pump device 50 beingcoupled with the droplet dispenser device 10 for aspirating a second portion of the suspension sample into the droplet dispenser device 10. Preferably, the droplet dispenser device 10 is adapted fordispensing single particle droplets on the target substrate 30. Furthermore, a method of isolating particles from a suspension sample is described.
Description
technical field [0001] The invention relates to a particle separation device configured for separating particles, in particular biological cells, cell aggregates, cell components or biological macromolecules, from a particle suspension sample. Furthermore, the invention relates to a method for separating particles from a sample of a particle suspension. The invention finds application in the field of processing particle suspensions such as biological samples, in particular for processing and recovering single biological cells or cell subpopulations, eg for culture or molecular analysis purposes. Background technique [0002] In the fields of biochemistry and biomedicine, there is interest in identifying rare cells within a larger background cell population, e.g. identifying specific cells in a blood sample that are indicative of disease (such as tumor cells) or that can be used for further cell processing . In general, identifying cells in a mixture of cells, such as a cel...
Claims
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IPC IPC(8): G01N1/28G01N1/38C12M1/00
CPCG01N1/28G01N1/38C12M23/02C12M47/04G01N2001/385B01L3/0241B01L3/0293B01L2400/0478B01L2400/0487B01L3/021B01L3/0265B01L2400/04B01L2400/0622G01N35/1009G01N2035/1034G01N2035/1058
Inventor 德里克·博格达诺夫埃里克·周迈克尔·威尔逊阿里埃勒·劳伦·格林菲尔德乔舒亚·坎特隆-布鲁斯
Owner 西尼昂有限公司