Unlock instant, AI-driven research and patent intelligence for your innovation.

Near infrared spectrum method for detecting BRAF V600E mutation

A near-infrared spectroscopy and spectroscopy technology, applied in the field of human gene mutation detection, can solve the problems of dyeing failure, time-consuming, high cost, etc.

Active Publication Date: 2020-02-07
CHONGQING MEDICAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in IHC-microscopy, IHC is a multi-step process that is susceptible to staining failures due to various factors, including all negatives, all positives, too dark background, well-stained positive controls but unstained positive samples , uneven dyeing intensity
Furthermore, in microscopy, the accuracy of mutation detection is severely limited by the pathologist's experience due to the subjectivity and complexity of morphological methods
On the other hand, polymerase chain reaction (PCR) and gene sequencing have at least the disadvantages of cumbersome operations, time-consuming and high costs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Near infrared spectrum method for detecting BRAF V600E mutation
  • Near infrared spectrum method for detecting BRAF V600E mutation
  • Near infrared spectrum method for detecting BRAF V600E mutation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 A near-infrared spectroscopy method for detecting BRAF V600E mutations in paraffin sections based on wild-type and mutant types of colorectal cancer

[0050] 1. Collection of samples

[0051] A total of 104 paraffin sections of colorectal cancer tissues from different patients were collected and recorded, including 52 slices of BRAF V600E wild type and 52 slices of BRAF V600E mutant.

[0052] 2. Spectral measurement

[0053] After preheating the spectrometer for 2 hours and passing the calibration, set the spectral measurement parameters to a resolution of 8cm -1 , Scan times 64 times, scan range 12000 ~ 4000cm -1 , cover the special infrared transmission and reflection spectrum measurement accessory for glass slide sample preparation over the slice (slide upward) placed on the detection window of the integrating sphere to completely cover the slice, measure the near-infrared transmission and reflection spectrum of each slice, each Before the second scan, t...

Embodiment 2

[0072] Example 2 A near-infrared spectroscopy method for detecting BRAF V600E mutations in dewaxed sections based on wild-type and mutant colorectal cancer

[0073] 1. Collection of samples

[0074] A total of 104 dewaxed sections of colorectal cancer tissues from different patients were collected and recorded, including 52 sections of BRAF V600E wild type and 52 sections of BRAF V600E mutant.

[0075] 2. Spectral measurement

[0076] With embodiment 1.

[0077] 3. Extraction and modeling of spectral characteristic variables

[0078] (1) Selection of spectral pretreatment scheme

[0079] In order to make the built model have excellent predictive performance, various spectral preprocessing techniques including NP, MSC, SNV, FD, SD, SGS, and NDS were screened and combined, see models 1-10 in Table 2. The results show that when the obtained spectra are not preprocessed, the prediction performance of the built model is the best, as shown in model 1 in Table 2.

[0080] (2) Se...

Embodiment 3

[0091] Example 3 A near-infrared spectroscopy method for detecting BRAF V600E mutations in HE-stained sections based on the wild-type and mutant types of colorectal cancer

[0092] 1. Collection of samples

[0093] A total of 104 HE-stained sections of colorectal cancer tissues from different patients were collected and recorded, including 52 sections of BRAF V600E wild type and 52 sections of BRAF V600E mutant.

[0094] 2. Spectral measurement

[0095] With embodiment 1.

[0096] 3. Extraction and modeling of spectral characteristic variables

[0097] (1) Selection of spectral pretreatment scheme

[0098] In order to make the built model have excellent predictive performance, various spectral preprocessing techniques including NP, MSC, SNV, FD, SD, SGS, and NDS were screened and combined, see models 1-10 in Table 3. The results show that when the obtained spectra are not preprocessed, the prediction performance of the built model is the best, as shown in model 1 in Table ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a near infrared spectrum method for detecting BRAF V600E mutation. The method comprises the following steps: collecting BRAF V600E wild type and mutant type cancer tissue slices, measuring near infrared spectra of the cancer tissue slices, processing spectral data by adopting a chemometrics method, and establishing a model for detecting the BRAF V600E wild type and mutant type by adopting a pattern recognition analysis method; then taking cancer tissue slices with unknown BRAF V600E mutation, collecting spectrums according to the same method and carrying out spectral data processing, and finally performing predicting by applying the established model. The method disclosed by the invention can be used for detecting the wild type and mutant type of the cancer tissue BRAF V600E sensitively, specifically, accurately, durably, simply, conveniently, quickly, nondestructively and inexpensively; the method is suitable for various cancer tissues possibly having BRAF V600E mutation, is also suitable for tissue forms of multiple links in the process from storage to pathological diagnosis of pathological tissues, and can be used for clinical auxiliary diagnosis.

Description

technical field [0001] The invention relates to a detection method of human gene mutation, more specifically, a near-infrared spectrum analysis method for detection of BRAF V600E mutation. Background technique [0002] Murine sarcoma viral oncogene homolog B1 (v-raf murine sarcoma viral oncogenehomolog B1, BRAF) is an important serine / threonine protein kinase, which participates in the mitogen-activated protein kinase (mitogen- Activated protein kinase (MAPK) signaling pathway regulates cell proliferation, differentiation and apoptosis. BRAF gene mutations are common in malignant tumors such as colorectal cancer, thyroid cancer, and melanoma. More than 30 types of BRAF mutations have been found, among which V600E mutation is considered to be the most common, which leads to the 600th valerian in the protein product. Amino acid (V) is replaced by glutamic acid (E). [0003] Detection of BRAF V600E mutation in cancer diagnosis (references: Guoren Deng, Ian Bell, Suzanne Crawl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/359G01N21/3563G16B40/10G16B20/50
CPCG01N21/359G01N21/3563G16B40/10G16B20/50
Inventor 范琦张雪王娅兰杨洋
Owner CHONGQING MEDICAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com