Preparation method of blueberry probiotic powder for middle aged and elderly people
A probiotics, middle-aged and elderly technology, applied in bacteria, food ultrasonic treatment, food science and other directions, can solve problems such as reducing the functional effect of probiotics, fruits and vegetables themselves, and unfavorable consumers' health, to avoid chemical changes, good effects, and avoidance. burden effect
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Embodiment 1
[0026] The present invention provides a kind of preparation method of middle-aged and elderly blueberry probiotic powder, comprising the following steps:
[0027] Step 1, under aseptic condition, dissolve the freeze-dried bacterial powder of Bacillus natto in sterilized physiological saline, and activate it statically at 37°C for 30 minutes;
[0028] Step 2, culture Bacillus natto based on sterilization at 121°C for 20 minutes, then cool to 37°C, inoculate statically activated strains according to 10% of the medium volume, and culture statically in an anaerobic box at 37°C for 12 hours; Centrifuge the good bacterial solution at 4000rpm for 15min to collect the bacterial sludge;
[0029] Step 3, adding 2% glycerin, 15% skimmed milk powder, 2% gelatin, and 12% trehalose to the bacteria slime in sequence, pre-freezing at -80°C for 12 hours, and then freeze-drying to obtain probiotic powder;
[0030] Step 4: Select fresh blueberries to remove rotten and diseased fruits, wash them...
Embodiment 2
[0035] The present invention provides a method for preparing probiotic powder of middle-aged and aged blueberries. The difference from the examples is that the sonic crushing power is 650W, the temperature is 38°C, and the ultrasonic time is 15min. 2; the detection results of active ingredients in the blueberry probiotic powder that the present embodiment obtains are as shown in table 1.
[0036] Table 1 Detection of active ingredients in blueberry probiotic powder
[0037] Example Anthocyanin content Bacillus natto protein sugar content 1 1.2mg / g 0.27g / g 4.42mg / g 5.7% 2 1.3mg / g 0.26g / g 4.53mg / g 5.9%
Embodiment 3
[0039] The present invention provides a method for preparing probiotic powder of middle-aged and aged blueberries. The difference from Example 1 is that in step 4, only a beater is used for crushing. Detect the dissolved substances in the serum obtained in step 3 of Example 1 and Example 2, as shown in Table 2.
[0040] Table 2
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[0042]
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