Preparation method of pectolinarin reference substance

Abstract

The invention discloses a preparation method of a pectolinarin reference substance. In particular, the method of the present invention comprises the steps of: (i) providing an alcohol extract of pectolinarin; (ii) dissolving the alcohol extract by using a NaOH aqueous solution, separating the alcohol extract by using a polyamide column through an eluent, and performing concentrating to obtain a semi-finished product of the pectolinarin; (iii) dissolving the semi-finished product of the pectolinarin, and separating the semi-finished product by utilizing a medium-pressure preparation column so as to obtain a crude product of the pectolinarin; (iv) carrying out alkali dissolution and acid precipitation on the crude product of the pectolinarin, filtering to obtain a solid, carrying out hot dissolution and cold precipitation on the solid by using methanol, and carrying out suction filtration to obtain a pure product of the pectolinarin; and (v) concentrating and freeze-drying the pure product to obtain the pectolinarin reference substance. The method is stable in process, convenient to operate, high in separation efficiency, low in cost, economical and environmentally friendly, and hashigh economic value and academic value.

Description

technical field [0001] The invention belongs to the technical field of modernization of traditional Chinese medicine, and relates to a preparation method of a chemical reference substance of traditional Chinese medicine. Specifically, the invention provides a preparation method of a reference substance of salicyloside leaf glycoside. Background technique [0002] Large thistle is the dry aboveground part of Cirsium japonicum Fisch.ex DC., which is a plant in the Compositae family. The aboveground part is harvested when the flowers bloom in summer and autumn, removed from impurities, and dried in the sun. The chemical components of thistle mainly include triterpenes and steroids, volatile oils, flavonoids and flavonoid glycosides, long-chain enynols, organic acids and other compounds. Clinically, thistle is sweet in taste, slightly bitter, cool in nature, and has the functions of "eliminating congestion, generating new blood, stopping vomiting blood, stopping nosebleeds and e...

AI Technical Summary

Problems solved by technology

[0005] At present, there are few domestic literature reports on the separation and purification of leaf glycosides from Salmonella lanceolata. In addition, leaf glycosides from Salmonfish lanceolata have been listed as standards in the 2015 edition of the Pharmacopoeia, and the demand for them is increasing. Very poor, it is very difficult to separate the monomer from medicinal materials, and the supply in the market is seriously insufficient. Therefore, it is very necessary to separate and purify the reference substance of the leaf glycosides from thistle, which has wide application value

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