Nano protective agent for improving stress resistance of crops and preparation method thereof
A protective agent and stress resistance technology, applied in the field of nano protective agent for improving crop stress resistance and its preparation field, can solve the problems of physiological race variation of pathogens, loss of resistance of crop varieties, environmental non-point source pollution, etc. resistance, improved cold resistance properties, improved nano-performance effects
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Embodiment 1
[0029] Example 1: Preparation and anti-powder mildew effect of nano protective agent
[0030] 1.1. Diatomite and attapulgite (100-200 mesh, purchased from Mingguang Guoxing Attapulgite Co., Ltd.) were subjected to intermittent irradiation treatment modification with low-energy ion beam (10-30keV, 1-1000kGy), treatment 10 minutes, stop for 5 minutes, and the total effective irradiation time is 20 minutes to obtain modified diatomite and attapulgite.
[0031] 1.2. Mix the modified diatomite and attapulgite in water with a mass ratio of 4:1. After drying, a porous nanocomposite material is obtained, which is used as the carrier of the nanoprotective agent.
[0032] 1.3. Mix melatonin (purchased from Sigma Reagent Co., Ltd.), karrikins (purchased from Sigma Reagent Co., Ltd.) and trehalose powder (purchased from Bioengineering (Shanghai) Co., Ltd.) and the nanocomposite material obtained in step 1.2 in water thoroughly, An aqueous solution of nanoprotective agent was prepared, wh...
Embodiment 2
[0035] Example 2: Preparation of nanoprotective agent and characteristics of drought resistance, cold resistance and salt resistance and powdery mildew resistance
[0036] 2.1. Preparation of nanoprotective agent
[0037] 2.1.1. Intermittently irradiate diatomite and attapulgite (100-200 mesh) with low-energy ion beam (10-30keV, 1-1000kGy), treat for 8 minutes, stop for 3 minutes, the total effective The irradiation time reached 30 minutes to obtain modified diatomite and attapulgite.
[0038] 2.1.2. Mix the modified diatomite and attapulgite in water with a mass ratio of 4:1, and after drying, a porous nanocomposite material is obtained, which is used as the carrier of the nanoprotective agent.
[0039] 2.1.3 The melatonin, karrikins and trehalose powders are fully mixed in water with the nanocomposite obtained in step 2.1.2 to prepare a nanoprotective agent, wherein the content of the composite nanomaterial is 1.2wt%, and the final concentration of melatonin is 1.2 wt%. Th...
Embodiment 3
[0056] Example 3: Preparation of nanoprotective agent and anti-cadmium properties
[0057] 3.1. Use low-energy ion beam (10-30keV, 1-1000kGy) to irradiate diatomite and attapulgite (100-200 mesh) intermittently, treat for 5 minutes, stop for 2 minutes, the total effective irradiation When the time reaches 40 minutes, modified diatomite and attapulgite are obtained.
[0058] 3.2. Mix the modified diatomite and attapulgite in water evenly at a mass ratio of 4:1, and after drying, a porous nanocomposite material is obtained, which is used as the carrier of the nanoprotective agent.
[0059] 3.3. Fully mix melatonin, karrikins and trehalose powder with the nanocomposite obtained in step 3.2 in water to prepare a nanoprotective agent, wherein the content of the composite nanomaterial is 2.5wt%, and the final concentration of melatonin is 200μM , the final concentration of karrikins was 10 nM, and the trehalose content was 0.1 wt%, where wt% was relative to the total weight of the ...
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