Test strip for detecting bifenthrin and application of test strip

A technology of bifenthrin and test strips, which is applied in the field of test strips for detecting bifenthrin, can solve problems such as soil pollution, excessive concentration, and inability to act as a drug barrier, achieving high sensitivity, long shelf life, The effect of short detection times

Inactive Publication Date: 2020-02-28
成都市房屋安全事务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the southern region, the drug is often used to prevent and control termites. When the content of bifenthrin is lower than 15ppm, it does not meet the sta...

Method used

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  • Test strip for detecting bifenthrin and application of test strip
  • Test strip for detecting bifenthrin and application of test strip
  • Test strip for detecting bifenthrin and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of test strips for detection of bifenthrin

[0036] The preparation method of the test strip mainly includes the following steps:

[0037] 1) Preparation of a conjugate release pad sprayed with bifenthrin monoclonal antibody-colloidal gold marker;

[0038] 2) Prepare a reaction membrane with a detection line coated with bifenthrin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0039] 3) Assemble the prepared combination release pad, reaction membrane, sample absorbent pad, water absorbent pad and PVC bottom plate prepared in 1) and 2) into test strips.

[0040] The following is a step by step description:

[0041] 1. Preparation of bifenthrin hapten

[0042] Take 0.322g of compound a, add 30ml of methanol to dissolve it, clarify it, and label it as solution A, take 0.155g of compound b, add 2ml of water to dissolve, add to solution A, stir at room temperature for 2h, stop the reaction, remove methanol, ...

Embodiment 2

[0074] Example 2 Detection of Bifenthrin Residues in Samples

[0075] 1. Test with test strips

[0076] Use a pipette to draw 3 drops of the sample solution to be tested vertically and drop 3 drops into the sample hole. When the liquid flows, start timing, react for 5-10 minutes, and determine the result.

[0077] 2. Analyze the test results

[0078] Read the results through the colloidal gold analyzer ("Analyzer"):

[0079] Negative (-): indicates that the concentration of the substance to be tested in the sample is lower than the detection limit;

[0080] Positive (+): indicates that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit;

[0081] Invalid: indicates that the test needs to be retested.

Embodiment 3

[0082] Example 3 Sample testing example

[0083] 1. Detection limit test

[0084] Take samples of blank soil and door and window holes, add bifenthrin to the final concentration of 7.5, 15, and 30 mg / kg respectively, take test strips for testing, and repeat the measurement for each sample three times.

[0085] When using test strips to test soil and door and window samples, when the concentration of bifenthrin is 7.5mg / kg, the analyzer shows negative; when the concentration of bifenthrin is 15, 30mg / kg, the analysis The meter shows positive.

[0086] 2. False positive rate and false negative rate test

[0087] Take the soil with a known bifenthrin content of 15mg / kg, 20 positive samples for door and window holes, and 20 samples for each soil with a content of less than 15mg / kg, and 20 negative samples for door and window holes. Use three batches of test strips to test and qualitatively Calculate the negative-positive rate of the two methods of determination and quantitative determinat...

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PUM

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Abstract

The invention discloses a test strip for detecting bifenthrin and an application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), wherein the reaction film is provided with a detection line (5) coated with a bifenthrin hapten-carrier protein conjugate and a quality control line (6) coated with a goat-anti-mouse antibody, and the conjugate release pad (2) is sprayed with a bifenthrin monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting bifenthrin residues in masonry walls such as soil, door and window holes and the like by using the bifenthrin test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

Description

Technical field [0001] The present invention relates to a test strip for detecting bifenthrin and its application, in particular to a colloidal gold test strip for detecting bifenthrin, which is particularly suitable for connecting masonry walls such as soil, doors and windows, etc. Rapid qualitative and quantitative detection of phenthrin residues. Background technique [0002] Bifenthrin, also known as Uranus, Zongfenling, and Bifenning, is a highly efficient synthetic pyrethroid insecticide and acaricide with contact killing and stomach toxicity, no systemic or fumigation effects. It has a wide insecticidal spectrum and has a good control effect on mites. It acts quickly and does not move in the soil. It is a high-efficiency and low-toxic pesticide, but its residues can still cause poisoning of humans and animals and seriously endanger human health. [0003] Because different proportions have different effects, and at the same time, sanitary insecticides are chemical products ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/53
CPCG01N33/5308G01N33/558G01N33/577
Inventor 张亮贾慧娴熊先贵彭凯俊万宇平王思忠曾小虎崔海峰崔娜
Owner 成都市房屋安全事务中心
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