High-throughput screening method for progesterone response defect infertility human sperm samples

A screening method and high-throughput technology, applied in the medical field, can solve the problems of inefficient screening of sterile samples, cumbersome steps, and long time consumption, and achieve stable results, high sensitivity, and low photobleaching effects
CN110873706AInactive Publication Date: 2020-03-10NANTONG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANTONG UNIVERSITY
Publication Date
2020-03-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a high-throughput screening method for progesterone response defect infertility human sperm samples. According to the invention, healthy reproductive men are used as normal control, a male patient with infertility caused by unknown reasons is used as a research object, the effect of progesterone among different human sperm samples on the concentration ([Ca<2+>]i) of free Ca<2+> in head cells is observed and screened at high throughput, human sperm infertility samples with progesterone response defects are efficiently and rapidly screened while the dosing time differenceis avoided, the influence of progesterone on the human sperm function and the molecular mechanism of progesterone are further explored, and meanwhile, the clinical significance of sperm membrane progesterone receptor function defects in evaluation of male infertility causes is expected to be illuminated.
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Description

technical field

[0001] The invention belongs to the field of medical technology, and in particular relates to a high-throughput screening method for progesterone response-deficient infertile human sperm samples. Background technique

[0002] After entering the female reproductive tract, sperm can quickly respond to changes in the external physical and chemical environment, and undergo a series of physiological processes and functional changes such as capacitation, hyperactivation, and acrosome reaction, before finally combining with egg cells to complete fertilization. Progesterone is the most important physiological hormone that regulates the function of mammalian mature sperm. It is secreted by cumulus cells and presents a concentration gradient distribution in the female reproductive tract. The physiological function acts as an inductive feature, guiding the sperm to successfully swim to the egg cell, and avoiding the premature hyperactivation of the sperm and the loss of...

Claims

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