Broad-band achromatic multi-focus microscopic imaging optical system

Abstract

The application discloses a wide-band achromatic multi-focus microscopic imaging optical system, a microscope, an achromatic lens group, a multi-focus diffractive optical element, an image detector and a chromatic aberration correction device. The microscope imaging is placed on the front focal plane of the achromatic lens group, the multi-focus diffractive optical element is arranged on the relay plane of the achromatic lens group, and the image detector is arranged on the back focal plane of the achromatic lens group. The multi-focus diffractive optical element generates multiple focal points in the direction of the optical axis and arranges each image sequentially in the meridian direction, so that the image detector can image multiple object surfaces of the sample at the same time; its focal length and off-axis amount are based on the image distance interval , The optical parameters of the microscope and achromat lens group are determined. The chromatic aberration correction device is used to correct the image chromatic aberration of the non-zero diffraction order produced by the multi-focus diffractive optical element by adjusting the incident angle of the refractive optical element. The application has a simple optical path, is easy to adjust and is applicable to a wide band, can effectively reduce chromatic aberration, and improve the resolution of microscopic imaging.

Description

technical field [0001] The present application relates to the technical field of microscopic imaging, in particular to a wide-band achromatic multi-focus microscopic imaging optical system. Background technique [0002] Microscopic imaging systems play a decisive role in the development of modern biomedicine, but for a long time, optical microscopy systems have been facing a problem: high lateral resolution and large axial depth of field cannot be achieved at the same time, until the confocal technology was proposed, this problem It was solved very well. Confocal technology uses point-by-point illumination and pinhole diaphragms to remove stray light from the spatial non-focal plane to obtain axial high resolution, and obtains the depth information of the object by scanning the object layer by layer. Using this microscopic imaging method to observe static objects can not only obtain high-precision lateral resolution, but also obtain three-dimensional information with a larg...

AI Technical Summary

Problems solved by technology

However, the prism needs to be designed and processed according to the characteristic size of the multi-focus diffractive optical element, and multiple sets of prisms need to be designed and processed for backup. The universality is not strong, and the cost is high.

In addition, the grating contains diffraction elements, which makes it difficult to efficiently utilize the incident light, which makes it difficult to ensure high-quality imaging for objects with low luminous flux

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