Thrombin aptamer sensor based on Ti3C2 and preparation method of thrombin aptamer sensor
An aptamer sensor and thrombin technology, applied in instruments, scientific instruments, fluorescence/phosphorescence, etc., can solve the problems of complex and time-consuming reactions, difficult preparation and storage of antibodies, etc., and achieve the effect of fast reaction, easy storage, and simple synthesis
Active Publication Date: 2020-03-24
JILIN UNIV
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Abstract
The invention relates to a thrombin aptamer sensor based on Ti3C2 and a preparation method of the thrombin aptamer sensor which belong to the technical field of biological analysis and detection, andsolve the technical problems that an antibody used in a traditional thrombin detection method is difficult to prepare and store, and the related reactions are complex and time-consuming. The thrombinaptamer sensor based on Ti3C2 is composed of a single layer of Ti3C2 and a fluorescein-labeled thrombin aptamer combined to the surface of the Ti3C2, and the sequence of the fluorescein labeled thrombin aptamer is 5 '-FAM-GGT TGG TGT TGT TGG-3'. According to the invention, the thrombin sensor is constructed by adopting a Ti3C2 material, so that the application of Ti3C2 in the biological field is broadened, and an excellent nano detection platform is constructed; the thrombin aptamer is used as a thrombin recognition agent, and compared with a traditional antibody, the thrombin aptamer is low in price, is easy and convenient to synthesize, is easy to store and is rapid in reaction. The Ti3C2-based thrombin aptamer sensor prepared by the preparation method provided by the invention has the characteristics of high specificity and high sensitivity, and the detection limit of thrombin is 2.5*10-11 mol/L.
Application Domain
Fluorescence/phosphorescence
Technology Topic
AntibodyFluorescein +5
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