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Anti-oxidation method of biological material and biological material

A biological material and anti-oxidation technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as changes, and achieve the effects of convenient application, long storage time, and saving rescue time

Inactive Publication Date: 2020-03-27
PEIJIA MEDICAL TECH SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application provides an anti-oxidation method of biological materials and biological materials, in order to solve the technical problem that biological materials are easily affected by the external environment and change during the preservation process of biological materials in a dry state

Method used

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  • Anti-oxidation method of biological material and biological material
  • Anti-oxidation method of biological material and biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Antioxidant treatment:

[0036] The reducing agent is sodium borohydride, and the buffer is PBS solution. Weigh 0.5 g of sodium borohydride, dissolve it in 100 ml of absolute ethanol, then put the porcine aortic valve into it, shake it at room temperature for 24 hours, and rotate at 50 rpm. It should be noted that the porcine aortic valve can be subjected to anti-calcification treatment before anti-oxidation treatment, and the porcine aortic valve used here has been subjected to anti-calcification treatment.

[0037] 2. Dried:

[0038] The anti-oxidation-treated porcine aortic valve was shaken and washed three times with PBS solution, 10 minutes each time, and then freeze-dried with a freeze dryer, and the freeze-dried porcine aortic valve was stored in a box.

Embodiment 2

[0040] 1. Antioxidant treatment:

[0041] Sodium triacetoxyborohydride was selected as the reducing agent, and PBS solution was selected as the buffer. Measure 100ml of PBS solution containing 0.5% sodium triacetoxyborohydride, adjust the pH value to 7.40 with sodium hydroxide solution, pour it into a container, add 3 pieces of bovine pericardium with a diameter of 50mm, and place it in a refrigerator at 4°C. Refrigerate for 48 hours. It should be noted that the bovine pericardium can be cross-linked before anti-oxidation treatment, and the bovine pericardium used here has been cross-linked with glutaraldehyde.

[0042] 2. Dried:

[0043]The bovine pericardium was taken out, and washed three times with 80% / 20% ethanol / normal saline solution on a shaker at room temperature, each time for 15 minutes.

[0044] Soak the cleaned bovine pericardium with 95% ethanol solution for 2 hours, take it out, soak it in absolute ethanol for 2 hours, replace it with new absolute ethanol sol...

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Abstract

The invention discloses an anti-oxidation method of a biological material and the biological material. The anti-oxidation method comprises the following steps: under preset conditions, carrying out anti-oxidation treatment on the biological material by using a reducing agent, and storing the biological material subjected to the anti-oxidation treatment in a dry state. The biological material is subjected to the anti-oxidation treatment by adopting the reducing agent, so that the biological material has certain anti-oxidation capacity, and the material can be well protected from unpredictable changes in an uncontrollable external environment. Meanwhile, the biological material subjected to anti-calcification treatment is stored in a dry state. The requirement for storage conditions is low when the biological material is stored in the dry state, the storage time is longer, and the biological material has the capacity of tolerating adverse environments. Meanwhile, the biological materialstored in the dry state can be directly used after complete package is unsealed, rinsing and assembling are not needed, application is more convenient, and rescue time is saved.

Description

technical field [0001] The application belongs to the technical field of biological material processing, and in particular relates to an anti-oxidation method of biological material and biological material. Background technique [0002] Biomaterials are a type of natural or synthetic special functional materials that are used to contact and interact with living systems, and can diagnose, treat, replace, repair, or induce regeneration of their cells, tissues, and organs, also known as biomedical materials. . Biological materials include artificial synthetic materials and natural materials; there are single materials, composite materials, and hybrid materials that combine living cells or natural tissues with inanimate materials. Biomaterials themselves are not drugs, and their therapeutic pathway is characterized by direct combination and interaction with biological organisms. [0003] At present, the preservation method of biological materials usually adopts dry state prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3687A61L27/3691A61L27/3625A61L27/3604A61L27/362A61L27/3629A61L27/3608A61L27/50A61L2430/40
Inventor 潘孔荣张一张坤汪文涛
Owner PEIJIA MEDICAL TECH SUZHOU
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