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Apple pectin heteropolysaccharide for improving intestinal flora diversity and preparation method thereof

A technology of apple pectin and intestinal flora, applied in the field of food processing, can solve the problems of slow fermentation rate of intestinal microorganisms, low degradation ratio, low proportion of neutral sugar, etc., so as to improve the structure of intestinal microbial flora, fermentation Moderate rate, pH-lowering effect

Active Publication Date: 2021-03-30
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The probiotic effect of this pectin is still not fully exerted, mainly due to the following reasons: one is that the molecular weight of pectin is too high, and the fermentation rate of intestinal microorganisms is slow, especially the degradation ratio in the cecum is low; The high degree of digestion limits the enzymatic cleavage efficiency of polygalacturonase PG secreted by intestinal microorganisms, which also reduces the fermentation rate; the third is that the proportion of neutral sugar is relatively low, which leads to a small number of advantages that belong to a large number of proliferation , the result is that the flora proliferation species is relatively single

Method used

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  • Apple pectin heteropolysaccharide for improving intestinal flora diversity and preparation method thereof
  • Apple pectin heteropolysaccharide for improving intestinal flora diversity and preparation method thereof
  • Apple pectin heteropolysaccharide for improving intestinal flora diversity and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The preparation method of the apple pectin heteropolysaccharide which improves the diversity of intestinal flora comprises the following steps:

[0030] Step 1, dissolving apple powder in water for enzymolysis, concentrating and purifying under reduced pressure to obtain a pectin extract, wherein the preparation method of apple powder is as follows: peel and core the apples, beat the apples, then dry, pulverize, Pass through a 40-mesh sieve to obtain apple powder. Those skilled in the art can use one of α-amylase, protease, amyloglucose, and cellulase for enzymolysis according to conventional techniques; for purification, conventional techniques can be used for ethanol precipitation.

[0031] Step 2, adding pectin methylesterase and pectin acetylesterase to the pectin extract for a directional enzyme digestion, then adding galacturonanase and pectin lyase for a secondary directional enzyme digestion, and then adding Polyrhamnogalacturonase, xylosylgalacturonase, galacta...

Embodiment 2

[0034] The preparation method of the apple pectin heteropolysaccharide which improves the diversity of intestinal flora comprises the following steps:

[0035] Step 1, dissolving apple powder in water for enzymolysis, concentrating and purifying under reduced pressure to obtain a pectin extract, wherein the preparation method of apple powder is as follows: peel and core the apples, beat the apples, then dry, pulverize, Pass through a 60-mesh sieve to obtain apple powder. Those skilled in the art can use one of α-amylase, protease, amyloglucose, and cellulase for enzymolysis according to conventional techniques; for purification, conventional techniques can be used for ethanol precipitation.

[0036] Step 2, adding pectin methylesterase and pectin acetylesterase to the pectin extract for a directional enzyme digestion, then adding galacturonanase and pectin lyase for a secondary directional enzyme digestion, and then adding Polyrhamnogalacturonase, xylosylgalacturonase, galacta...

Embodiment 3

[0039] Step 1, dissolving apple powder in water for enzymolysis, concentrating and purifying under reduced pressure to obtain a pectin extract, wherein the preparation method of apple powder is as follows: peel and core the apples, beat the apples, then dry, pulverize, Pass through a 50-mesh sieve to obtain apple powder. Those skilled in the art can use one of α-amylase, protease, amyloglucose, and cellulase for enzymolysis according to conventional techniques; for purification, conventional techniques can be used for ethanol precipitation.

[0040] Step 2, adding pectin methylesterase and pectin acetylesterase to the pectin extract for a directional enzyme digestion, then adding galacturonanase and pectin lyase for a secondary directional enzyme digestion, and then adding Polyrhamnogalacturonase, xylosylgalacturonase, galactanase, and arabinanase were subjected to three directional digestions to obtain a modified pectin extract. The conditions for one directional digestion wer...

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Abstract

The invention discloses a preparation method of an apple pectin heteropolysaccharide for improving diversity of intestinal florae. The preparation method comprises the following steps: I, dissolving apple powder into water, performing enzymolysis, performing vacuum concentration, and performing purification so as to obtain a pectin extract; II, adding pectin methylesterase and pectin acetylase into the pectin extract, performing primary oriented digestion, adding polygalacturonase and pectate lyase, performing secondary oriented digestion, further adding polyrhamnogalacturonases, xylogalact uronase, galactan esterases and arabanase, and performing third oriented digestion so as to obtain a modified pectin extract; and III, performing purification treatment on the modified pectin extract, so as to obtain the apple pectin heteropolysaccharide. Through three times of oriented digestion, the apple pectin heteropolysaccharide which is high in neutral polysaccharide ratio, low in methyl esterification degree and low in acetylation degree can be obtained, the apple pectin heteropolysaccharide is capable effectively improving diversity of intestinal florae, and the microorganism fermentation degradation rate in intestines within 24 hours is greater than 80%.

Description

technical field [0001] The invention relates to the field of food processing. More specifically, the present invention relates to an apple pectin heteropolysaccharide that improves the diversity of intestinal flora and a preparation method thereof. Background technique [0002] Pectin is a kind of acid heteropolysaccharide rich in galacturonic acid in plant cell wall, and it is an important kind of water-soluble dietary fiber. Apple pectin is a typical heteropolysaccharide, in addition to polygalacturonic acid (HG) structure, it also contains rhamnogalacturonan type I (RG-I), rhamnogalacturonan II Type (RG-II), xylogalacturonan (XGA) and other structural units, these structural units are connected by at least 20 kinds of glycosidic bonds. Compared with galacturonic acid, these monosaccharides that make up pectin are called neutral sugars, and even intestinal microorganisms cannot easily degrade them. Among them, the monosaccharide composition of the rhamnogalacturonan typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04C08B37/06
CPCC08B37/0045C08B37/0048C12P19/04C12P19/14
Inventor 易建勇毕金峰赵圆圆吴昕烨李旋陈芹芹马千程
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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