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Extraction Method of abamectin

A technology of abamectin and extraction method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem of high sewage discharge, unfavorable environmental protection, volatile toluene working environment, large consumption of organic solvents, etc. It can reduce the production cost and discharge of sewage, avoid the use of toluene as a solvent, and improve the extraction efficiency.

Active Publication Date: 2020-04-07
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process uses toluene to dissolve the extract and then washes it with hot water to remove polar impurities such as sugar. The process steps are cumbersome, the consumption of organic solvents is large, the cost is high, and toluene is volatile, resulting in poor working environment, and the amount of sewage discharge is not conducive to Environmental friendly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take Abamectin Fermented Liquid 50m 3 , the titer was 7339 μg / ml, and the total billion was 367.0 billion.

[0024] Dynamic ultrasonic extraction: the above-mentioned avermectin fermentation broth is filtered and pulverized to obtain the avermectin scum. 7.6 times the weight of ethanol was added to the fungus residue, and the dynamic ultrasonic extraction method was used to extract at a temperature of 70°C for 35 minutes, and the extraction frequency was 5kHz. The extract was concentrated under reduced pressure to obtain the extract.

[0025] Cloud point extraction method for desugaring: Add APEO solution with a weight concentration of 25g / L to the extract according to the liquid-solid ratio of 50:1, stir and mix evenly, 500W ultrasonic treatment for 10min, and centrifuge to obtain the supernatant. Add 0.5% NaCl solution (5mol / L) of the extract weight to the supernatant, and mix well. When heated in a water bath at 54°C for about 30 minutes, the solution was clearly ...

Embodiment 2

[0029] Take Abamectin Fermented Liquid 50m 3 , the titer was 7520 μg / ml, and the total billion was 376.0 billion.

[0030] Dynamic ultrasonic extraction: the above-mentioned avermectin fermentation broth is filtered and pulverized to obtain the abamectin scum. Add 8.5 times the weight of ethanol to the fungus residue, and use dynamic ultrasonic extraction method to extract at a temperature of 80°C for 45 minutes, and the extraction frequency is 7kHz. The extract was concentrated under reduced pressure to obtain the extract.

[0031] Cloud point extraction method for desugaring: Add APEO solution with a weight concentration of 30g / L to the extract according to the liquid-solid ratio of 80:1, stir and mix evenly, 500W ultrasonic treatment for 10min, and centrifuge to obtain the supernatant. Add NaCl solution (6mol / L) 1% times the weight of the extract to the supernatant, and mix well. When heated in a water bath at 65°C for about 26 minutes, the solution was clearly divided i...

Embodiment 3

[0035] Take Abamectin Fermented Liquid 50m 3 , the titer was 8051 μg / ml, and the total billion was 402.6 billion.

[0036] Dynamic ultrasonic extraction: the above-mentioned avermectin fermentation broth is filtered and pulverized to obtain the fungus residue. Add 12 times the weight of ethanol to the fungus residue, and use the dynamic ultrasonic extraction method to extract at a temperature of 90°C for 50 minutes, and the extraction frequency is 8kHz. The extract was concentrated under reduced pressure to obtain the extract.

[0037]Cloud point extraction method for desugaring: Add APEO solution with a weight concentration of 40g / L to the extract according to the liquid-solid ratio of 100:1, stir and mix evenly, 500W ultrasonic treatment for 10min, and centrifuge to obtain the supernatant. Add 0.85% NaCl solution (8 mol / L) of the weight of the extract to the supernatant, and mix well. When heated in a water bath at 60°C for about 35 minutes, the solution was clearly divid...

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Abstract

The invention relates to an extraction method of abamectin. The process comprises the following steps: firstly, extracting abamectin residue by a dynamic ultrasonic extraction method, concentrating the obtained extracting solution into an extract, desugaring by extracting the extract through a cloud point extraction method, centrifuging for phase separation to obtain a surfactant phase and a waterphase, performing back extraction on the surfactant phase by a microwave-assisted extraction method, crystallizing the obtained back extract liquor by a gradient cooling crystallization method, carrying out centrifugal separation, and drying to obtain the abamectin finished product. The dynamic ultrasonic extraction is combined with the cloud point extraction for desugar, the microwave-assisted method for back extraction and the gradient cooling crystallization for extraction and purification of abamectin, so that the use of organic solvents is reduced, production costs are lowered, the environmental protection index is improved, and the process steps are simplified, and the extraction efficiency, extraction yield and product weight of abamectin are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic purification, in particular to an extraction method of abamectin. Background technique [0002] Abamectin is a commonly used pesticide in my country, and it has the effect of repelling and killing nematodes, insects, and mites. The traditional abamectin extraction process is to filter the abamectin fermentation liquid through a plate frame, crush the bacteria residue, and then extract it with ethanol. After the extract is evaporated to dryness, it is dissolved in toluene, washed with hot water, and concentrated to obtain a paste. The product was dissolved in methanol, crystallized for 2 to 3 times, and then centrifuged and dried to obtain the product. This process uses toluene to dissolve the extract and then washes it with hot water to remove polar impurities such as sugar. The process steps are cumbersome, the consumption of organic solvents is large, the cost is high, and toluene is volatil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06
CPCC07H17/08C07H1/06Y02A50/30
Inventor 梁丽娟刘鹏于荣张智圣田刚卫
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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