A kind of in vitro dynamic cell culture device and culture method thereof

A cell culture and dynamic technology, applied in tissue cell/virus culture devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problem that the cell incubator cannot meet the dynamic culture of cells or tissues, and achieve cell growth The effect of good condition, various clamping methods, and easy operation

Active Publication Date: 2020-11-06
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problem that the existing cell incubator cannot satisfy the dynamic culture of cells or tissues in the simulated in vivo mechanical microenvironment, the present invention proposes an in vitro dynamic cell culture device for growth and differentiation under controllable mechanical stimulation and its cultivation method

Method used

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  • A kind of in vitro dynamic cell culture device and culture method thereof
  • A kind of in vitro dynamic cell culture device and culture method thereof
  • A kind of in vitro dynamic cell culture device and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Using the in vitro dynamic cell culture device designed by the present invention (such as figure 1 RMSC-bm cells were observed under the dynamic mechanical stimulation conditions of stretching rate of 10 mm / min, stretching elongation rate of 20%, stretching for 72 hours (i.e. 3 days), and continuous stretching for 2 hours per day. Growth state on helical fiber bundles.

[0117] Dynamic in vitro cell culture steps

[0118] Step 1, sterilizing the fiber bundles;

[0119] Soak the spiral fiber bundles in 75% alcohol for half an hour to achieve disinfection, take out the spiral fiber bundles and place them in a sterile ultra-clean bench (Model Thermo 1389) and turn on UV lamps for sterilization for half an hour to obtain sterile fiber bundles.

[0120] Step 2, pre-culturing fiber bundles;

[0121] Place sterile fiber bundles in a six-well plate (model COSTAR 3516) with an inoculation volume of 40 μL and a density of 1 × 10 6 cell / mL rat bone marrow mesenchymal stem cell...

Embodiment 2

[0153] Using the in vitro dynamic cell culture device designed by the present invention (such as figure 1 ), set the stretching rate to 30 mm / min, the tensile elongation rate was 100%, stretched for 24 hours (i.e. 1 day), and continuously stretched for 24 hours a day under dynamic mechanical stimulation conditions, and observed NIT-3T3 cells. Growth state on helical fiber bundles.

[0154] Step 1, sterilizing the fiber bundles;

[0155] Soak the spiral fiber bundles in 75% alcohol for half an hour to achieve disinfection, take out the spiral fiber bundles and place them in a sterile ultra-clean bench (Model Thermo 1389) and turn on UV lamps for sterilization for half an hour to obtain sterile fiber bundles.

[0156] Step 2, pre-culturing fiber bundles;

[0157] Place sterile fiber bundles in a six-well plate (model COSTAR 3516) with an inoculation volume of 20 μL and a density of 1 × 10 6 Cell / mL of embryonic fibroblast suspension was fixed for 1 h, and then 2 mL of cell cu...

Embodiment 3

[0168] Using the in vitro dynamic cell culture device designed by the present invention (such as figure 1 ), set the stretching rate to 5 mm / min, the stretching elongation rate to 50%, the stretching time to be 48 hours (ie, 2 days), and to observe the RMSC-bm under the dynamic mechanical stimulation conditions of continuous stretching for 10 hours per day. Growth state of cells on helical fiber bundles.

[0169] Step 1, sterilizing the fiber bundles;

[0170] Soak the spiral fiber bundles in 75% alcohol for half an hour to achieve disinfection, take out the spiral fiber bundles and place them in a sterile ultra-clean bench (Model Thermo 1389) and turn on UV lamps for sterilization for half an hour to obtain sterile fiber bundles.

[0171] Step 2, pre-culturing fiber bundles;

[0172] Place sterile fiber bundles in a six-well plate (model COSTAR 3516) with an inoculation volume of 40 μL and a density of 1 × 10 6 cell / mL rat bone marrow mesenchymal stem cell suspension was f...

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Abstract

The invention discloses an in-vitro dynamic cell culture device and a culture method thereof. A connecting piece A (6) and a connecting piece B (7) in the device are used for supporting a culture dish(1); a second clamp assembly (3) is installed on a driving assembly (4) installed on an upper panel of a base (5); and the driving assembly (4) is used for providing reciprocating motion of the second clamp assembly (3). A first clamp assembly (2) and a second clamp assembly (3) are symmetrically installed on the two sides of the culture dish (1). A drive motor trigger signal F1 output by a control system (100) is sent to the driving assembly (4) to realize stretching of the assembly. The device can be placed in a cell culture box for dynamic culture, a simple cell culture optimization function is performed under the condition of not changing the cell culture device, and the cost is reduced. In-vivo cells are simulated to be in a mechanical microenvironment, a cell culture method under mechanical stimulation is established, and a positive effect on development of cells and tissue engineering scaffolds and tissue culture methods is realized.

Description

technical field [0001] The invention relates to a culturing device for simulating the microenvironment of cells in vivo, more particularly, to an in vitro dynamic cell culturing device and its culturing method for studying the growth and differentiation of cells under certain mechanical stimulation. Background technique [0002] In recent years, with the development of cell culture technology, transplantation technology and biomaterial technology, clinical medicine has stepped into a new stage of "regenerative medicine" - tissue engineering. Tissue engineering is to inoculate relevant tissue cells cultured in vitro on a biomaterial with excellent compatibility and degradability to form a cell-material composite scaffold, and then implant the cell-material composite scaffold into the damaged tissue. While the biomaterials of cells are degraded and absorbed by the body over time, the cells continue to proliferate, migrate and differentiate to form new tissues to achieve the pu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/22C12M1/00
CPCC12M23/10C12M35/04
Inventor 赵勇王玉亮王雅琼曾炳霖王女
Owner BEIHANG UNIV
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