Lactobacillus rhamnosus capable of regulating and controlling relative abundance of acinetobacter in intestinal tracts

A technology of Lactobacillus rhamnosus and intestinal health, applied in the field of microorganisms, can solve the problems of hindering the treatment of Acinetobacter infection and the lack of pertinence of Acinetobacter, achieve good oligosaccharide utilization ability, improve intestinal health, and have great application prospects Effect

Inactive Publication Date: 2020-04-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned probiotics lack specificity to Acinetobacter. At present, there is still no probiotic strain that can significantly inhibit Acinetobacter in the human intestinal tract, which undoubtedly hinders the treatment of Acinetobacter infection

Method used

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  • Lactobacillus rhamnosus capable of regulating and controlling relative abundance of acinetobacter in intestinal tracts
  • Lactobacillus rhamnosus capable of regulating and controlling relative abundance of acinetobacter in intestinal tracts
  • Lactobacillus rhamnosus capable of regulating and controlling relative abundance of acinetobacter in intestinal tracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Screening and strain identification of Lactobacillus rhamnosus

[0049] 1. Screening

[0050] Taking the feces of newborns from the Department of Obstetrics and Gynecology of Huaxing Hospital in Dagang, Tianjin as samples, they were serially diluted to 10 times with sterile normal saline. -6 , and then take 100 μL dilution gradient of 10 -4 、10 -5 、10 -6 Spread the diluted liquid on the MRS solid medium, culture at 37°C for 48 hours, observe and record the colony shape; pick the colonies of different shapes on the MRS solid medium to separate them by streaking, and after culturing at 37°C for 48 hours, pick again Take the single colonies of different shapes on the MRS solid medium and separate them by streaking until the pure single colonies with the same shape are obtained; pick the pure colonies on the MRS solid medium and inoculate them in 5mL MRS liquid medium, and incubate at 37°C for 18h; Take 1mL of the bacterial solution in a sterile centrifuge tu...

Embodiment 2

[0053] Embodiment 2: the cultivation of Lactobacillus rhamnosus

[0054] Lactobacillus rhamnosus (Lactobacillus rhamnosus) CCFM1039 was inoculated on MRS solid medium and cultured at 37°C for 48 hours. The colonies were observed and found to be round, white, opaque and smooth.

Embodiment 3

[0055] Example 3: Tolerance of different Lactobacillus rhamnosus to simulated gastrointestinal fluid

[0056] Specific steps are as follows:

[0057] 1. Tolerance of different Lactobacillus rhamnosus to simulated gastric juice

[0058] Lactobacillus rhamnosus (Lactobacillus rhamnosus) CCFM1039, Lactobacillus rhamnosus (Lactobacillus rhamnosus) LR1, Lactobacillus rhamnosus LR2, Lactobacillus rhamnosus LR3, Lactobacillus rhamnosus (Lactobacillus rhamnosus) LR4 and Lactobacillus rhamnosus (Lactobacillus rhamnosus) LR5 were respectively inserted into MRS liquid medium and cultured at 37°C for 18 hours, then the cells were collected by centrifugation, and the collected cells were washed with normal saline, washed After the end, the cells were collected by centrifugation again, and the collected cells were resuspended in the physiological saline containing 3g / L pepsin at pH 3 (pH adjusted by HCl) to the bacterial solution OD 600 If it is 5.0, take 1mL of bacterial liquid to count ...

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Abstract

The invention discloses lactobacillus rhamnosus capable of regulating and controlling relative abundance of acinetobacter in intestinal tracts, which belongs to the technical field of microorganisms and medicines. The lactobacillus rhamnosus CCFM1039 screened by the invention can be used for improving the intestinal health, which is specifically reflected in that (1) fructo-oligosaccharide, xylooligosaccharide and galactooligosaccharide can be utilized, and the lactobacillus rhamnosus CCFM1039 has a good oligosaccharide utilization capability; (2) various short-chain fatty acids such as aceticacid, propionic acid and butyric acid can be produced through in-vitro metabolism; and (3) the relative abundance of Acinetobacter in intestinal tracts can be reduced, so that the lactobacillus rhamnosus CCFM1039 has a huge application prospect in the preparation of products (such as foods, medicines or health care products) for improving the health of the intestinal tracts.

Description

technical field [0001] The invention relates to a Lactobacillus rhamnosus capable of regulating the relative abundance of Acinetobacter in the intestinal tract, and belongs to the technical field of microorganisms and the technical field of medicine. Background technique [0002] The intestinal tract is not only the digestive site of the human body, but also the largest immune organ of the human body. It is rich in huge and complex microbial colonies. There are more than 800 species and more than 7,000 strains of bacteria, showing great diversity at the subspecies or strain level. sex. Intestinal microbes have an important impact on human health. They can help host cell metabolism, provide nutrition for intestinal epithelial cells, enhance immune function, and resist the invasion of foreign microorganisms. Under normal circumstances, intestinal microorganisms and the human body are in a symbiotic state. The microorganisms absorb the nutrients needed for growth from the host...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/00C12R1/225
CPCA23L33/135A61P1/00A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/175A23V2200/3204
Inventor 陈卫田丰伟翟齐啸陆文伟吉梦馨沈旭丹于雷雷赵建新张灏
Owner JIANGNAN UNIV
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