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Simoa kit of preeclampsia risk prediction marker PIGF and application thereof

A technology of eclampsia markers and kits, which can be used in biological testing, measuring devices, and biological material analysis. It can solve the problems of large sample size, insufficient sensitivity, and complicated operations, and achieve wide detection range, high sensitivity, and short time-consuming Effect

Pending Publication Date: 2020-04-14
ZHENGZHOU UNIV
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Problems solved by technology

[0008] The present invention proposes a Simoa kit of pre-eclampsia marker PIGF and its application, in order to solve technical problems such as complex operation, large sample size requirement and insufficient sensitivity in the prior art

Method used

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  • Simoa kit of preeclampsia risk prediction marker PIGF and application thereof
  • Simoa kit of preeclampsia risk prediction marker PIGF and application thereof

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Experimental program
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Embodiment

[0031] The Simoa kit for detecting PIGF content in human blood provided by the present invention comprises 2.7 μm magnetic beads coated with capture antibody, PIGF standard, biotinylated detection antibody, streptavidin-β-galactoside Enzyme (SBG) solution, fluorogenic substrate solution and sample diluent, and electronic documentation of the assay method.

[0032] In the present invention, the raw materials of magnetic beads, capture antibodies, standards, detection antibodies, biotinylation reagents, SBG and fluorescent substrates are purchased; wherein the capture antibodies are monoclonal antibodies, specifically, mouse anti-human PIGF monoclonal antibodies Cloned antibody; the detection antibody is a polyclonal antibody, specifically a rabbit anti-human PIGF polyclonal antibody.

[0033] In the capture antibody-coated magnetic bead scheme of the present invention, the preparation process is as follows: first, the stored antibody is exchanged through an ultrafiltration tube...

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Abstract

The invention belongs to the field of immunodetection technology and preeclampsia risk prediction, and particularly relates to a Simoa kit of a marker PIGF and application thereof. The Simoa kit comprises capture antibody coated magnetic beads, a PIGF standard substance, a biotinylated detection antibody, an SBG solution, a fluorescent substrate solution and a sample diluent. According to the Simoa kit for detecting the PIGF protein content in the human blood based on the Simoa platform and the double-antibody sandwich method, the PIGF protein content in the human blood can be qualitatively and quantitatively detected; the method has the characteristics of high sensitivity, high flux, less sample size, automation, short time, simplicity and convenience in operation, wide detection range and the like.

Description

technical field [0001] The invention belongs to the field of immune detection technology and risk prediction of preeclampsia, in particular to a Simoa kit of a preeclampsia marker PIGF and its application. Background technique [0002] Placental growth factor (PIGF) is a member of the VEGF (vascular endothelial growth factor) subfamily. PIGF is a key molecule during angiogenesis and embryogenesis. The major source of PIGF during pregnancy is placental trophoblast cells. Preeclampsia is a pregnancy disorder characterized by episodes of high blood pressure and large amounts of protein in the urine. This condition often appears after the 20th week of pregnancy. In severe cases, ruptured red blood cells, low platelet count, impaired liver function, kidney insufficiency, swelling, and shortness of breath due to fluid buildup in the lungs or visual disturbances may occur. Preeclampsia increases the risk of poor outcomes for both mother and baby. If left untreated, it can lead...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/475G01N2800/368
Inventor 李朝辉蔡齐勇张琳屈凌波
Owner ZHENGZHOU UNIV
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