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Intermittent spraying type plant bioreactor and culture method

A bioreactor and spray technology, which is applied in the field of plant tissue culture, can solve the problems of high shear force of liquid medium and damage to plant tissue, etc., and achieve the effects of fully utilizing waste, reducing waste, and avoiding vitrification

Pending Publication Date: 2020-04-17
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Purpose of the invention: for the problems existing in the prior art, the present invention provides a kind of intermittent spray type plant bioreactor and cultivation method, the reactor and cultivation method of the present invention have solved liquid culture medium shear force is big, causes plant tissue injury problem

Method used

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  • Intermittent spraying type plant bioreactor and culture method
  • Intermittent spraying type plant bioreactor and culture method

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Experimental program
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Effect test

Embodiment 1

[0026] like figure 1 Shown, a kind of intermittent spray type plant bioreactor, comprises growth chamber 1, spray device 2, liquid storage chamber 3, peristaltic pump 4 and time controller 5; Growth chamber 1, liquid storage chamber 3, spray device 2 and the peristaltic pump 4 are sequentially connected in circulation; the growth chamber 1 is cylindrical, and a small hole is arranged on the top, and is connected to the first sterile air filter 102; at the bottom of the growth chamber 1, the sealing ring and bayonet or screw thread are used to connect with the accumulation liquid. The funnel-shaped structure 104 is sealed and connected, and the connecting portion between the growth chamber 1 and the funnel-shaped structure 104 is provided with a detachable seedling-raising tray 103, and the seedling-raising tray 103 is provided with several small holes; the top of the growth chamber 1 is provided with a cover 101 with a diameter of 1 / 5-1 / 3 of the diameter of the top of the grow...

Embodiment 2

[0030] The cultivation method of intermittent spray type plant bioreactor, comprises the steps:

[0031] (1) Put the seedling tray 103 in the growth chamber 1, seal and connect the first sterile air filter 102 and the funnel-shaped structure 104, tightly cover the lid 101 with the spray device 2, and connect it for connecting the lid 2, one end of the first aseptic joint 6 and one end of the second aseptic joint 303 used to connect the funnel-shaped structure 104, wrap up the connected structure as a whole, sterilize it with high temperature and high pressure, and cool it for later use;

[0032] (2) Prepare the culture solution, pour the culture solution into the liquid storage chamber 3, plug the silica gel plug 301, and connect the second sterile air filter 304, connect the first sterile joint 6 and the second sterile air filter respectively The other end of the joint 7 is wrapped up as a whole and then sterilized by high temperature and high pressure damp heat, and cooled f...

Embodiment 3

[0037] Utilize the cultivation of the Dendrobium nobile tissue culture seedling of intermittent spray reactor, adopt the intermittent spray reactor of embodiment 1 and the cultivation method of embodiment 2:

[0038](1) Each structure in the growth chamber is assembled and bandaged, sterilized by high temperature and high pressure at 121°C, and cooled for later use.

[0039] (2) Prepare 1L culture medium (recipe: 1 / 2MS + 1g / L Huabao 2 + 80mL / L coconut milk + 20g / L sucrose pH6.0), pour it into the liquid storage chamber, plug in the silicone plug and connect well Each subsidiary structure is sterilized by high temperature and high pressure after being bandaged, and then cooled for later use.

[0040] (3) In the ultra-clean workbench, open the cover of the growth chamber and inoculate the 1-2 cm high sterile Dendrobium nobile tissue culture seedlings obtained by proper amount of solid culture on the seedling tray of the growth chamber.

[0041] (4) Connect the aseptic interface...

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Abstract

The invention discloses an intermittent spraying type plant bioreactor and a culture method. The intermittent spraying type plant bioreactor comprises a growth chamber, a spraying device, a liquid storage chamber, a peristaltic pump and a time controller, wherein the growth chamber, the liquid storage chamber, the spraying device and the peristaltic pump are sequentially and circularly connected;the time controller is connected with the peristaltic pump; the peristaltic pump is connected to the spraying device arranged inside a cover arranged above the growth chamber and the liquid storage chamber through a guide pipe and a sterile joint; a seedling tray is arranged inside the growth chamber; and a funnel-shaped structure is arranged at the bottom of the growth chamber, and is connected with the liquid storage chamber through a guide pipe and a sterile joint. The spraying type plant bioreactor and the culture method provided by the invention have the advantages that nutrient substances are sufficiently utilized; waste is reduced; tissue culture seedlings healthily grow; vitrification is avoided; program control and mechanical operation are used, so that the intermittent spraying type plant bioreactor and the culture method are suitable for realizing large-scale culture; and high flux of the culture is realized by connecting a plurality of growth chambers in series.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an intermittent spray type plant bioreactor and a culture method. Background technique [0002] Plant tissue culture technology was founded in the middle of the 20th century. After decades of development, it has been widely used in plant gene function verification, molecular breeding, plant developmental physiology, plant mutant research and hybrid breeding, detoxification and rejuvenation of plant varieties, Research and industry aspects such as germplasm resource purification, seedling propagation and factory agriculture. At present, the most mainstream plant tissue culture technology at home and abroad is still the traditional tissue culture bottle / agar solid culture technology. This technology is mature, easy to operate, and suitable for a wide variety of plants, but the solidified agar also limits the rapid dispersion and balanced distribution of nut...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 丁小余张本厚牛志韬薛庆云刘薇
Owner NANJING NORMAL UNIVERSITY
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