Method for synergistically fermenting non-grain feed by bacteria and enzymes

A non-grain feed and collaborative fermentation technology, which is applied in animal feed, animal feed, food processing, etc., can solve the problems of large variation, underutilization, and low feeding value of nutrients, and achieve the reduction of ammonia concentration and energy saving. Equipment input cost, effect of high mycotoxin content

Inactive Publication Date: 2020-04-17
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country has a vast territory, rich products, and a wide variety of non-grain feed resources. However, due to its complex composition, nutritional imbalance, various anti-nutritional factors and mycotoxins, it cannot be used directly or must be used in limited quantities without treatment. Low value, affected by factors such as the source of origin, processing and storage conditions, etc., the nutrient content varies greatly, the quality is unstable, etc., and it has not been fully utilized in animal husbandry production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Prepare the composite bacteria liquid required for fermentation: get 55 parts of lactic acid bacteria, 10 parts of saccharomyces and 35 parts of Bacillus subtilis to prepare composite bacteria, and first add water of 60% of the volume of the fermenter to the fermenter, and then add the water according to the per ton Water is mixed with 0.2kg of composite bacteria and 60kg of molasses and then propagated in a fermenter at a temperature of 36°C for 5 days, so that the concentration of the composite bacteria after propagation is ≥1.0×10 8 CFU / mL;

[0022] (2) Prepare the compound enzyme required for fermentation: get 20 parts of glucose oxidase, 30 parts of acid protease and 50 parts of cellulase and mix uniformly, and prepare the compound enzyme whose enzyme activity is 42,000 U / g;

[0023] (3) Preparation of non-grain feed fermentation substrate: take 40 parts of corn husks, 30 parts of corn germ meal and 30 parts of Artemisia argyi leaves, pulverize and mix to prepa...

Embodiment 2

[0028] (1) Prepare the composite bacteria liquid required for fermentation: get 22 parts of lactic acid bacteria, 13 parts of saccharomyces and 65 parts of Bacillus subtilis to prepare composite bacteria, and first add water of 80% of the volume of the fermenter to the fermenter, and then according to the rate per ton Add 2.0kg of composite bacteria and 20kg of molasses to the water and mix them in a fermenter at a temperature of 37°C for 3 days, so that the concentration of the composite bacteria solution after propagation is ≥1.0×10 8 CFU / mL;

[0029] (2) Prepare the compound enzyme required for fermentation: get 40 parts of glucose oxidase, 35 parts of acid protease and 25 parts of cellulase and mix uniformly, and prepare the compound enzyme whose enzyme activity is 34,000 U / g;

[0030] (3) Preparation of non-grain feed fermentation substrate: take 70 parts of corn husks, 20 parts of corn germ meal and 10 parts of Artemisia argyi leaves, pulverize and mix to prepare a ferme...

Embodiment 3

[0035] (1) Prepare the composite bacteria liquid required for fermentation: get 20 parts of lactic acid bacteria, 60 parts of saccharomyces and 20 parts of Bacillus subtilis to prepare composite bacteria, and first add water with 70% of the volume of the fermenter in the fermenter, and then according to the rate per ton Add 1.0kg of composite bacteria and 40kg of molasses to the water and mix them in a fermenter at a temperature of 36.5°C for 4 days, so that the concentration of the composite bacteria solution after propagation is ≥1.0×10 8 CFU / mL;

[0036] (2) Prepare the compound enzyme required for fermentation: get 30 parts of glucose oxidase, 36 parts of acid protease and 34 parts of cellulase and mix uniformly to prepare a compound enzyme whose enzyme activity is 38,000 U / g;

[0037] (3) Preparation of non-grain feed fermentation substrate: take 50 parts of corn husks, 25 parts of corn germ meal and 25 parts of Artemisia argyi leaves, pulverize and mix to prepare a ferme...

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Abstract

The invention provides a method for synergistically fermenting non-grain feed by bacteria and enzymes. The method comprises the following steps: using lactic acid bacteria, saccharomycetes and bacillus subtilis to prepare a compound bacteria solution in a fermentation tank; using glucose oxidase, acid protease and cellulose to prepare a compound enzyme, using corn bran, corn germ meal and wormwoodto prepare a fermentation substrate, using the fermentation substrate, the compound enzyme and compound bacteria solution to prepare a fermented feed, and using the fermented feed to prepare the final non-grain fermented feed in a breathing bag. According to the method, the breathing bag provided with a one-way valve is used, gas generated by anaerobic fermentation of the feed only comes out butnot goes in, and growth of beneficial bacteria and fermentation heat dissipation are facilitated; according to the method, the non-grain feed corn bran, the corn germ meal and the wormwood are fermented by utilizing a bacterium-enzyme synergistic technology, so that the nutritional value of the non-grain feed is improved, the animal growth performance is improved, the mycotoxin content is reduced,the beneficial bacterium content of the fermented feed is high, the animal immunity is improved, the ammonia gas concentration in a livestock and poultry breeding house is reduced, and non-grain feedresources are expanded for breeding industry.

Description

technical field [0001] The invention relates to a method for synergistically fermenting non-grain feed with bacteria and enzymes, and belongs to the technical field of solid-state fermented feed. Using corn bran, corn germ meal and mugwort leaves as non-grain feed fermentation substrates, lactic acid bacteria, yeast and Bacillus subtilis were multiplied to prepare compound bacterial liquid, and glucose oxidase, acid protease and cellulase were used to prepare compound enzymes. The non-grain fermented feed is prepared by mixing the compound bacterial solution, compound enzyme and non-grain feed, and put into a breathing bag with a one-way valve, and put a common feed packaging bag on the outside of the breathing bag. The fermented feed bags are stacked in a constant temperature sealed fermentation room, and the non-grain fermented feed product is prepared through constant temperature fermentation. The present invention utilizes bacteria-enzyme synergistic technology to ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K10/12A23K10/14A23L5/20
CPCA23K10/37A23K10/12A23K10/14A23L5/25A23L5/28Y02P60/87
Inventor 张晶于浩金永成房恒通沈景林周长海赵云丁雪梅史旭东
Owner JILIN UNIV
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