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Helicobacter pylori transporting and storing device and method

A technology of Helicobacter pylori and a storage device, applied in the field of microorganisms, can solve the problems of death of Helicobacter pylori, inconvenient disease research, and difficulty in survival, etc., and achieve the effects of improving transportation and preservation efficiency, improving survival rate, and easy operation.

Pending Publication Date: 2020-04-21
杭州金域医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because HP is a microaerophilic bacterium, the ambient oxygen requirement is 5%-8%, it is very sensitive to gas, and it is difficult to survive in the atmosphere or an absolute anaerobic environment, and ordinary culture medium is due to the Exposure of HP samples to natural gas resulted in the death of a large number of Helicobacter pylori, which brought great inconvenience to clinical testing and research on related diseases

Method used

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  • Helicobacter pylori transporting and storing device and method
  • Helicobacter pylori transporting and storing device and method
  • Helicobacter pylori transporting and storing device and method

Examples

Experimental program
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Effect test

Embodiment approach

[0059] As a possible implementation, the method for transporting and preserving Helicobacter pylori comprises the following steps:

[0060] (1) Place the Helicobacter pylori sample in the culture medium, place the gained culture medium on the fixed support of the culture tank, cover the tank cover tightly, and fix the tank body and the tank cover with a fixing device;

[0061] (2) The second exhaust port of the gas storage is connected with the air inlet of the aerator through a connecting pipe, and the first exhaust port of the aerator is connected with the aeration port of the culture tank through a vent pipe;

[0062] (3) Vacuumize the culture tank through the inflator, then fill the culture tank with gas for microaerophilic microorganism cultivation, and transport in cold chain;

[0063] (4) Inoculation and cultivation.

[0064] As a possible implementation, before sampling the Helicobacter pylori sample, it should be confirmed that the patient stopped taking antibacteria...

Embodiment 1

[0068] Embodiment 1 Helicobacter pylori delivery preservation method

[0069] 1) Patient preparation

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Abstract

The invention discloses a Helicobacter pylori transporting and storing device and method. The Helicobacter pylori transporting and storing device comprises a Helicobacter pylori culture tank, a gas storage device and an inflator, wherein the Helicobacter pylori culture tank comprises a tank body and a tank cover, the tank cover is provided with a gas inlet / outlet, and the gas inlet / outlet is provided with a one-way valve; a bracket is arranged in the tank body; a fixing device for fixing the tank cover is arranged on the edge of the upper part of the tank body; the inflator is provided with agas inlet and a first exhaust port; and a second exhaust port is formed in the gas storage device. The Helicobacter pylori transporting and storing device and method are easy and convenient to operate, interfaces do not need to be switched and a three-way valve is not needed in the gas exhaust / exchange process, and a proper micro-aerobic environment can be provided for the transporting and storingprocess of Helicobacter pylori samples.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a device and method for transporting and preserving Helicobacter pylori. Background technique [0002] Microaerophilic microorganisms have very high requirements on growth conditions, are very sensitive to the oxygen content in the environment, and often need to survive and grow in a specific range of oxygen content. Helicobacter pylori is one of the typical representatives of microaerophilic microorganisms. [0003] Helicobacter pylori (Hp) is an important pathogenic factor of gastritis, peptic ulcer and gastric cancer. In 1994, the World Health Organization designated Helicobacter pylori as a class I carcinogen, and it is currently the only prokaryotic pathogenic microorganism listed as a carcinogen. The diagnostic criteria for Helicobacter pylori use bacterial culture. Because HP is a microaerophilic bacterium, the ambient oxygen requirement is 5%-8%, it is ver...

Claims

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Application Information

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IPC IPC(8): C12M1/04C12M1/00C12N1/04C12R1/01
CPCC12M23/48C12M23/02C12M29/06C12M23/36C12M29/20C12N1/04
Inventor 廖雨琴刘军权单宇祝宇翀项伶俐冯莉亚
Owner 杭州金域医学检验所有限公司