Preparation method of protective liquid containing feline panleukopenia yolk antibodies
A leukopenia and yolk antibody technology, applied to medical preparations containing active ingredients, antibodies, medical preparations with no active ingredients, etc., can solve the problems of short storage period, inconvenient long-distance transportation, passive application, etc., and achieve The method is simple, guarantees the quality of antibodies, and promotes the effect of digestion
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[0023] Example 1
[0024] A preparation method of a protective solution containing feline pan-leukopenia egg yolk antibody, characterized in that: the protective solution includes components and their weight ratios: konjac gum 20g, purslane flavone 20g, sucrose 10g, Lactic acid bacteria 5g, phosphate buffer 800g.
[0025] A preparation method of a protective solution containing feline pan-leukopenia egg yolk antibody is characterized in that the steps of the protective solution preparation method are as follows:
[0026] 1. Preparation of protection solution
[0027] (1) Weigh konjac gum, portulaca flavonoids and sucrose by weight, add phosphate buffer solution, stir to dissolve, and then sterilize at 105°C for 10 minutes at high temperature and humidity to obtain solution A;
[0028] (2) Prepare lactic acid bacteria into bacterial solution B according to the weight ratio, and store it at 2-8°C for later use;
[0029] (3) Mix the solution A and the bacterial liquid evenly, and divide th...
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[0036] Example 2
[0037] A preparation method of a protective solution containing feline pan-leukopenia egg yolk antibody, which is characterized in that: the protective solution includes components and their weight ratios respectively: 40 g of konjac gum, 30 g of portulaca flavone, 30 g of sucrose, 10g of lactic acid bacteria, 1000g of phosphate buffer.
[0038] A preparation method of a protective solution containing feline pan-leukopenia egg yolk antibody is characterized in that the steps of the protective solution preparation method are as follows:
[0039] 1. Preparation of protection solution
[0040] (1) Weigh konjac gum, portulaca flavonoids and sucrose by weight, add phosphate buffer solution, stir to dissolve, and then sterilize at 120°C for 10 minutes at high temperature and high humidity to obtain solution A;
[0041] (2) Prepare lactic acid bacteria into bacterial solution B according to the weight ratio, and store it at 2-8°C for later use;
[0042] (3) Mix the solution ...
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[0049] Example 3
[0050] Preparation of yolk antibody for cat pan-leukopenia
[0051] 1. High immune egg production: select healthy, unimmunized laying hens, and use pan-leukopenia vaccine to immunize the chickens before laying eggs, including basic immunization, booster immunity, and booster immunity. The immunization interval is 8 days. The dosage is 1ml / head / time, and the high-immune eggs will be collected when the serum antibody titer reaches 1:1024;
[0052] 2. Clean and disinfect high-free eggs, collect and stir to obtain egg yolk homogenate;
[0053] 3. Add 1.5 times the volume of normal saline and 0.1% n-octanoic acid to the egg yolk homogenate obtained in step 2, and stir for 1.5 hours while adding. After leaving it at room temperature for 6 hours, centrifuge at 5500r / min for 30 minutes and discard. Precipitate, add 1.2% dextran sulfate to the supernatant, stir for 20 minutes, filter the supernatant with a cartridge filter with a pore size of 5μm and 1μm, and then concentra...
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