Nano bioremediation method for high-concentration polycyclic aromatic hydrocarbon contaminated site soil

A polycyclic aromatic hydrocarbon and bioremediation technology, which is applied in the restoration of polluted soil, fertilization methods, grain cultivation, etc., can solve the problems of long restoration period and low removal efficiency of bioremediation technology, shorten the restoration period and improve restoration efficiency. , will not cause the effect of secondary pollution

Active Publication Date: 2020-04-28
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: Facing the major demand for soil remediation of high-concentration PAHs-contaminated sites, and aiming at the above-mentioned deficiencies in the prior art, the present invention proposes a nano-bioremediation method for soils polluted by high-concentration polycyclic aromatic hydrocarbons, which can overcome bioremediation The technical removal efficiency is low and the restoration cycle is long, etc., to achieve efficient, green, economical and sustainable restoration of contaminated soil on the site

Method used

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  • Nano bioremediation method for high-concentration polycyclic aromatic hydrocarbon contaminated site soil
  • Nano bioremediation method for high-concentration polycyclic aromatic hydrocarbon contaminated site soil
  • Nano bioremediation method for high-concentration polycyclic aromatic hydrocarbon contaminated site soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: The strengthening effect of different sulfonated graphene additions on the removal of PAHs in soil by alfalfa

[0017] The soil collected from a contaminated site of a coking plant in Nanjing was used as the test soil to carry out indoor pot experiments. The collected soil was naturally air-dried to remove the gravel and animal and plant residues, passed through a 4 mm sieve and mixed thoroughly. The PAHs content in the soil was 344.48 mg / kg. 0.6 kg of soil for potting, 0.215 g of urea per kg of soil, Ca(H 2 PO 4 ) 2 ·H 2 O 0.285g and K 2 SO 4 0.188g. Add sulfonated graphene to the soil, set different addition levels (0.05, 0.1, 0.2, 0.5, 1.0, 5.0 mg / g), and repeat three times for each treatment. Use the following method to germinate alfalfa seeds: Soak alfalfa seeds in 0.5% sodium hypochlorite solution for surface disinfection, clean them after 10 minutes, then place them in 95% alcohol solution for surface sterilization for 10 minutes, and rinse the...

Embodiment 2

[0020] Example 2: The strengthening effect of different single-armed carbon nanotube additions on the removal of PAHs in soil by alfalfa

[0021] The soil collected from a contaminated site of a coking plant in Nanjing was used as the test soil to carry out indoor pot experiments. The collected soil was naturally air-dried to remove the gravel and animal and plant residues, passed through a 4 mm sieve and mixed thoroughly. The PAHs content in the soil was 344.48 mg / kg. 0.6 kg of soil for potting, 0.215 g of urea per kg of soil, Ca(H 2 PO 4 ) 2 ·H 2 O 0.285g and K 2 SO 4 0.188g. Add single-armed carbon nanotubes to the soil, set different addition levels (0.05, 0.1, 0.2, 0.5, 1.0, 5.0 mg / g), and each treatment has three replicates. Use the following method to germinate alfalfa seeds: Soak alfalfa seeds in 0.5% sodium hypochlorite solution for surface disinfection, clean them after 10 minutes, then place them in 95% alcohol solution for surface sterilization for 10 minut...

Embodiment 3

[0024] Example 3: The strengthening effect of different multi-armed carbon nanotube additions on the removal of PAHs in soil by alfalfa

[0025] The soil collected from a contaminated site of a coking plant in Nanjing was used as the test soil to carry out indoor pot experiments. The collected soil was naturally air-dried to remove the gravel and animal and plant residues, passed through a 4 mm sieve and mixed thoroughly. The PAHs content in the soil was 344.48 mg / kg. 0.6 kg of soil for potting, 0.215 g of urea per kg of soil, Ca(H 2 PO 4 ) 2 ·H 2 O 0.285g and K 2 SO 4 0.188g. Add multi-armed carbon nanotubes to the soil, set different addition levels (0.05, 0.1, 0.2, 0.5, 1.0, 5.0 mg / g), and each treatment has three replicates. Use the following method to germinate alfalfa seeds: Soak alfalfa seeds in 0.5% sodium hypochlorite solution for surface disinfection, clean them after 10 minutes, then place them in 95% alcohol solution for surface sterilization for 10 minutes...

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Abstract

The invention discloses a nano bioremediation method for high-concentration polycyclic aromatic hydrocarbon contaminated site soil. The method comprises the following steps: adding a carbon nanomaterial and a base fertilizer into the high-concentration polycyclic aromatic hydrocarbon contaminated site soil, uniformly spreading medicago sativa seeds into the contaminated soil, keeping the water content of the soil at 60% of the field water capacity, harvesting plants when medicago sativa grows to a flowering or maturation period, and removing the plants from the contaminated soil. According tothe method, the problems of low removal efficiency, long repair period and the like of a bioremediation technology can be solved; the advantages of the nanotechnology and polycyclic aromatic hydrocarbon pollutant degradation by plant rhizosphere are fully exerted; the remediation efficiency is improved, the remediation period is shortened, operation is easy, secondary pollution cannot be caused inthe remediation process, disturbance to soil is small, the economic cost is low, and thus efficient, environment-friendly, economical and sustainable remediation of the site contaminated soil is achieved, and the method has wide application prospects in the field of remediation of the high-concentration polycyclic aromatic hydrocarbon contaminated soil.

Description

technical field [0001] The invention belongs to the application and development of nanotechnology in the technical field of soil pollution remediation, and in particular relates to a nanobiological remediation method for high-concentration polycyclic aromatic hydrocarbon-contaminated soil. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of persistent organic pollutants composed of two or more benzene rings, which are ubiquitous in the environment. Due to the "three causes" effects of carcinogenicity, teratogenicity and mutagenicity, PAHs have been listed as priority pollutants by the United States and the European Union, among which 7 kinds of polycyclic aromatic hydrocarbons such as benzo[a]pyrene have also been included in the "China Environmental Priority Pollutants Blacklist". In recent years, with the rapid development of industrialization and urbanization, PAHs in my country's soil have been accumulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/105B09C2101/00B09C1/08A01G22/20A01C21/00A01D91/04
Inventor 任文杰滕应王慧敏
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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