Method for conveniently and quickly purifying dihydromyricetin from ampelopsis grossedentata leaves

A technology for dihydromyricetin and dihydromyricetin is applied in the field of convenient purification of dihydromyricetin, which can solve the problems of long time, high energy consumption and high cost, and achieve low solubility, low energy consumption and reduced use volume Effect

Active Publication Date: 2020-04-28
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the related method and technology of column chromatography separation and purification steps are combined, which results in significantly higher costs due to the need for more materials and organic solvents used for chromatography; while the use of hot water extraction combined with cooling recrystallization to remove impurities Purification method requires higher energy consumption and more (due to the recrystallization process) time and other costs

Method used

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  • Method for conveniently and quickly purifying dihydromyricetin from ampelopsis grossedentata leaves
  • Method for conveniently and quickly purifying dihydromyricetin from ampelopsis grossedentata leaves
  • Method for conveniently and quickly purifying dihydromyricetin from ampelopsis grossedentata leaves

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Embodiment 1

[0023] The present embodiment specifically provides a process of purifying and obtaining dihydromyricetin from the dried leaves of Snake-toothed Grape (mildew tea), which is based on the above-mentioned method of the present invention, and the specific steps are as follows:

[0024] (1) Pulverize the sample of Odontodon raisin leaf mold tea, pass through a 80-100 mesh sieve to obtain the dry powder of the material, then take 10 g of the above-mentioned dry powder sample and extract it with 40 ml of ethanol / water solution with a volume fraction of 70% at room temperature for 3 hours, filter Separated to obtain alcohol extract.

[0025] (3) Add 10ml of pure water to 30ml of alcohol extract, and then remove the alcohol solvent in the alcohol extract by distillation under reduced pressure in a water bath at 45 degrees Celsius. As the alcohol is gradually distilled out, the remaining solvent is mainly water A large amount of solids were gradually precipitated in the solution, left ...

Embodiment 2

[0028] The present embodiment specifically provides a process of purifying and obtaining dihydromyricetin from the dried leaves of Snake-toothed Grape (mildew tea), which is based on the above-mentioned method of the present invention, and the specific steps are as follows:

[0029] (1) Pulverize the sample of the dentata raisin leaf mold tea sample, pass through 80~100 mesh sieves to obtain the material dry powder, then get the above dry powder sample 10g and extract it with 40ml of methanol / water with a volume fraction of 82% at room temperature for 3 hours, centrifuge Separated to obtain alcohol extract.

[0030] (3) Add 10ml of pure water to the 30ml alcohol extract, and then remove the alcohol solvent in the alcohol extract by distillation under reduced pressure under the condition of a water bath at 40 degrees Celsius. A large amount of solids were gradually precipitated out of the solvent, left to cool to room temperature naturally, filtered to remove the water, and the...

Embodiment 3

[0033] The present embodiment specifically provides a process of purifying and obtaining dihydromyricetin from the dried leaves of Snake-toothed Grape (mildew tea), which is based on the above-mentioned method of the present invention, and the specific steps are as follows:

[0034] (1) Pulverize the sample of S. dentata raisin leaf mold tea, pass through a 80-100 mesh sieve to obtain the dry powder of the material, then take 10 g of the above-mentioned dry powder sample and extract it with 40 ml of methanol / water with a volume fraction of 90% at room temperature for 3 hours, and centrifuge Separated to obtain alcohol extract.

[0035] (3) Add 10ml of pure water to 30ml of alcohol-water extract, and then remove the alcohol solvent in the alcohol extract by distillation under reduced pressure under the condition of a water bath at 50 degrees Celsius. A large number of solids gradually precipitated out of the solvent, and after being left to cool to room temperature, the water w...

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Abstract

The invention discloses a method for conveniently and quickly purifying dihydromyricetin from ampelopsis grossedentata leaves. The method comprises the following steps: a, crushing ampelopsis grossedentata leaves, extracting the crushed ampelopsis grossedentata leaves by using alcohol/water with the volume fraction of 70-90%, and separating in a centrifuging or filtering manner to obtain an alcohol extracting solution, wherein the used alcohol is ethanol, methanol or isopropanol; b, adding water into the alcohol extracting solution, removing the alcohol solvent in the alcohol extracting solution through reduced pressure distillation under the water bath condition of 40-50 DEG C, gradually separating out solids from the remaining water-based solvent along with the gradual distillation removal of alcohol from the solvent, standing for cooling to room temperature, and then centrifuging or filtering to remove water to obtain a primarily extracted solid; and c, washing the primarily extracted solid with water, then carrying out suspension washing with an alcohol-containing ethyl acetate, alcohol-containing dichloromethane or alcohol-containing chloroform mixed solution, filtering to remove the washing solution, and drying to obtain dihydromyricetin, wherein the used alcohol is ethanol, methanol or isopropanol. The method has the advantages of simple operation, high extraction yieldand the like.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification methods for functional components of medicinal plants, and in particular relates to a technical method for conveniently purifying dihydromyricetin from the leaves of Grape dentata. Background technique [0002] Ampelopsis grossedentata (Ampelopsis grossedentata) is a woody vine of the genus Ampelopsis in the Vitaceae family. It is widely distributed in my country and has a medicinal history of hundreds of years. Due to its obvious pharmacological and health-care functions, it has been made into health-care tea drinks with typical regional characteristics, such as vine tea and mildew tea. Flavonoids are the main content of the active ingredients in Snake Grape, and the dihydromyricetin accounts for about 80% of its total flavonoids. According to literature reports, dihydromyricetin has various effects such as scavenging free radicals, controlling blood lipids and blood sugar, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/40C07D311/32
CPCC07D311/40C07D311/32
Inventor 谭建文钱涛徐巧林刘少博
Owner SOUTH CHINA AGRI UNIV
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