Fluorescent probe for detecting activity of acetylcholin esterase as well as synthesis method and application of fluorescent probe

Abstract

The invention discloses a fluorescent probe for detecting activity of acetylcholin esterase as well as a synthesis method and an application of the fluorescent probe. Based on the electrostatic interaction between MnO2 nanosheets and cationic derivatives (PBI-DM) of perylenetetracarboxylic diimide and the fluorescence spectrum characteristics of PBI-DM in the aggregation and dispersion process, the detection of the AChE activity is realized. The synthesized fluorescent probe PBI-DM-coated MnO2NS disclosed by the invention has a low detection limit of 0.24 mU / mL on the detection of AChE, has the advantages that the PBI-DM-coated MnO2NS is high in selectivity, interference of other low-concentration sulfhydryl compounds can be avoided, the PBI-DM-coated MnO2NS can be used for screening AChEinhibitors, and has great potential in the field of clinical diagnosis.

Description

technical field [0001] The invention belongs to the technical field of acetylcholinesterase activity detection, and relates to a method strategy for detecting acetylcholinesterase (AChE) activity. More specifically, the present invention not only relates to a MnO-based 2 Electrostatic interactions between nanosheets and cationic derivatives of perylenetetracarboximide (PBI-DM), and a new method for detecting AChE activity of PBI-DM's fluorescence spectral features during aggregation and dispersion, also related to the above Synthesis of fluorescent probes and their application in screening AChE inhibitors. Background technique [0002] Alzheimer's disease, one of the most common forms of dementia, is a chronic neurodegenerative disorder with symptoms including short-term memory loss, primary progressive aphasia, and confusion. Studies have shown that AChE can accelerate the abnormal aggregation of amyloid beta peptides to form Alzheimer's amyloid fibers in the central nerv...

AI Technical Summary

Problems solved by technology

Although the Ellman test is widely used, a large number of analytical studies have shown that it has certain shortcomings and limitations in the detection of AChE activity due to its false positive effect and poor precision.

In addition, conventional assays are less sensitive compared to luminescence assays, therefore, luminescence-sensing-based fluorescence assays have become the most attractive strategy for measuring AChE activity due to their ease of development, high sensitivity, good selectivity, and ease of operation. one

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