Microfluidic chip used for nucleic acid amplification detection and detection method

Abstract

The invention provides a microfluidic chip used for nucleic acid amplification detection and a detection method. The microfluidic chip comprises a substrate layer, a cover plate layer and a reversingvalve, wherein the cover plate layer is provided with a sample inlet, a purification and concentration chamber, a waste liquid outlet, a premixing chamber through hole and an outlet through hole; thesample inlet is connected to the waste liquid outlet and the premixing chamber through hole respectively after passing through the purification and concentration chamber through a flow channel; a porous material embedded with immunomagnetic beads is preset in the purification and concentration chamber; the reversing valve is arranged on a flow channel on the outlet side of the purification and concentration chamber to control a liquid in the purification and concentration chamber to flow to the waste liquid outlet or the premixing chamber through hole; the premixing chamber through hole is used for adding reactants to be mixed with the liquid from the purification and concentration chamber; the substrate layer is provided with a mixing flow channel and a constant-temperature amplificationchamber with a transparent area; and the premixing chamber through hole is connected with a constant-temperature amplification chamber through a mixing flow channel. By using the chip, purification, concentration, constant-temperature amplification and real-time fluorescence detection of nucleic acid can be realized in one step; and detection results of one or more target objects can be rapidly, simply, conveniently and reliably obtained.

Description

technical field [0001] The invention relates to molecular diagnosis technology, in particular to a microfluidic chip and a detection method for nucleic acid amplification detection. Background technique [0002] Since Manz and Widmer first proposed the concept of Miniaturized Total Analyze Systems, μTAS in the 1990s, microfluidic technology has been developed on the basis of microelectronics, micromechanics, bioengineering and nanotechnology. In the following decades, this field has rapidly developed into one of the most cutting-edge scientific and technological fields in the world, and its core technology is the microfluidic chip based on microfluidic technology, also known as Lab on chip. Microfluidic chips are widely used in the biomedical field because of their advantages such as low consumption, low cost, high throughput, and automatic operation. One of the important applications is molecular diagnostic technology based on microfluidic chips. [0003] Clinically, molec...

AI Technical Summary

Problems solved by technology

However, like the conventional nucleic acid detection technology, this technology cannot get rid of the shortcomings of time-consuming sample preparation and cumbersome operation, and does not meet the needs of rapid and low-cost clinical diagnosis

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