Bacillus with function of producing high-activity alginate lyase and application thereof

Abstract

The invention discloses bacillus with a function of producing high-activity alginate lyase and application thereof. The bacillus has a preservation number of CGMCC No.16120. The method for preparing alginate oligosaccharide by utilizing the bacillus comprises the following steps: 1, culturing a strain by activation and habituation; 2, acquiring a seed strain; 3, preparing a seed solution; 4, acquiring a crude enzyme of alginate lyase; 5, acquiring an initially enzyme hydrolyzed solution, namely adding the crude enzyme of alginate lyase into an algae pulp in a volume ratio of 1:100, performingenzyme hydrolysis in water bath at 40 DEG C for at least 24 hours, increasing the temperature to 120 DEG C, and performing inactivating for 1 hour; and 6, acquiring the alginate oligosaccharide, namely sequentially performing membrane filtration, concentration and freeze-drying on the initially enzyme hydrolyzed solution. The bacillus screened by the method can be used for preparing high-activityalginate lyase, has high conversion rate on primer algae pulp, and can improve production efficiency and reduce production cost when being used for preparing the alginate oligosaccharide.

Description

technical field [0001] The present invention relates to a Bacillus strain and application thereof, in particular to a Bacillus strain capable of producing high-activity alginate lyase ( Bacillus sp.) and its application in the preparation of brown algae oligosaccharides, belonging to the field of marine biotechnology. Background technique [0002] In recent years, with the expansion of the application field of alginate and the development of oligosaccharide degradation and separation technology, the biological activity of alginate oligosaccharide, the degradation product of alginate, has received great attention. Studies have found that in addition to physical and chemical characteristics such as strong solubility, high stability, safety and non-toxicity, brown algae oligosaccharides also have important physiological activities. It has been reported in the literature that brown algae oligosaccharides can promote plant root growth, antibacterial, antiviral activity, Heparin ...

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Problems solved by technology

[0004] The chemical-physical degradation method has problems such as difficult control of reaction conditions, high energy consumption, slow speed, low product recovery rate, difficult separation of target products, high cost, and serious environmental pollution in the post-processing process.

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