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Method for preparing animal-derived extracellular matrix polypeptide by using physical method

A technology of animal origin and exogenous matrix, which is applied to the preparation method of peptides, animal/human proteins, skin care preparations, etc., and can solve the problems of underutilization of the compatibility of various natural compound peptides, many chemical additives, single ingredients, etc. problems, to achieve the effect of rich active ingredients, long metabolic cycle, and long-lasting activity

Inactive Publication Date: 2020-05-08
上海中匡生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current small molecular peptides are chemically synthesized, with single ingredients, expensive unit prices, many chemical additives, and insufficient use of the compatibility of various natural compound peptides.

Method used

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  • Method for preparing animal-derived extracellular matrix polypeptide by using physical method
  • Method for preparing animal-derived extracellular matrix polypeptide by using physical method
  • Method for preparing animal-derived extracellular matrix polypeptide by using physical method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Embodiment 1 provides a method for physically preparing animal-derived extracellular matrix polypeptides, comprising the following steps:

[0083] (1) Clean the submucosa matrix of the porcine small intestine, divide it into strips with a length of 10 cm and a width of 2 cm, and heat-treat at 120° C. for 30 minutes to obtain tissue samples;

[0084] (2) Immediately place the above-mentioned tissue samples at -80°C for 24 hours, then thaw the tissue samples with warm water at 30°C at a mass ratio of 10:1, and repeat this process for 3 times to obtain the thawed tissue samples;

[0085] (3) Physically pulverize the thawed tissue samples at -196°C to prepare powder particles with a particle size of 200 mesh;

[0086] (4) Mix the above powder particles with pure water at a mass ratio of 3:100, and emulsify at 21000 rpm for 5 minutes to obtain a slurry;

[0087] (5) Heat the above slurry to 100°C for 30 minutes; then filter it with nylon gauze with a pore size of 37.5 μm, c...

Embodiment 2

[0090] Example 2 is basically the same as Example 1, the only difference is that the step (1) is to clean the pig peritoneal matrix, divide it into strips with a length of 10 cm and a width of 2 cm, and use 120 ° C high temperature treatment for 30 minutes to obtain tissue samples .

Embodiment 3

[0092] Example 3 is basically the same as Example 1, the only difference being that the high temperature in the step (1) is 140°C.

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Abstract

The invention relates to the technical field of a preparation method of a bioactive substance, in particular to a method for preparing animal-derived extracellular matrix polypeptide by using a physical method. The method comprises the following steps: 1 cleaning, cutting and carrying out high-temperature treatment on animal-derived tissue to obtain a tissue sample; 2 carrying out deep freezing treatment on the tissue sample, and thawing the tissue sample with water at 25-35 DEG C, and circulating the process for 3-5 times to obtain a thawed tissue sample; 3 physically crushing the thawed tissue sample to obtain powder particles; 4 dissolving the powder particles in water, and performing high-speed stirring and emulsification to obtain slurry; 5 heating the slurry for dissolution, carryingout suction filtration, collecting a filtrate, and performing drying to obtain powdery semi-finished product; and 6 carrying out irradiation treatment on the powdery semi-finished product to obtain the animal-derived extracellular matrix polypeptide. The ultralow-molecular-weight sterile complex polypeptide rich in type III collagen sequences is prepared with a combined physical preparation method, and high bioactive components are provided for skin whitening, caring and repair.

Description

technical field [0001] The invention belongs to the technical field of preparation methods of biologically active substances, and more specifically, the invention relates to a method for physically preparing animal-derived extracellular matrix polypeptides. Background technique [0002] Peptides are short chains of amino acids, which are precise protein fragments. Their molecules are only nanometer in size, and are easily absorbed by the stomach, blood vessels and skin. Small molecule peptides have higher skin permeability and are easier to be absorbed by human skin. At the same time, due to the small molecular weight to a certain extent, the biological activity has undergone a qualitative leap. The smaller the molecular weight of the peptide and the shorter the "amino acid chain", the easier it is to be absorbed and utilized by the body. [0003] Due to the unique biological personality and outstanding functional performance of small molecule peptides, it has a huge applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K14/78A23L33/18A61K8/64A61Q19/00A61Q19/02
CPCA23L33/18A23V2002/00A61K8/64A61Q19/00A61Q19/02C07K1/14C07K14/78A23V2250/55
Inventor 曾德文莫亚琴
Owner 上海中匡生物科技有限公司
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