Dual-wavelength common-path phase microscopic imaging measurement system based on F-P interferometer

Abstract

The invention provides an intracavity enhanced dual-wavelength common-path phase microscopic imaging measurement system based on an F-P interferometer. The system is characterized by being composed ofa laser light source 1, an optical attenuation sheet 2, an optical fiber coupler (LFC) 3, a single-mode optical fiber 4, an optical fiber collimator (FCL) 5, a beam expander 6, an F-P interferometer7, a to-be-measured object 8, a microscope objective 9, a CCD detection camera 10 and a computer 11. The system can be used for digital holography and refractive index measurement of tiny objects, andcan be widely applied to the field of refractive index three-dimensional microscopic imaging of various tiny objects.

Description

[0001] (1) Technical field [0002] The invention relates to a dual-wavelength co-channel phase microscopic imaging measurement system based on an F-P interferometer, which can be used for three-dimensional microscopic imaging of the refractive index inside cells and various tiny objects, and belongs to the technical field of optical imaging. [0003] (2) Background technology [0004] Micro-optical imaging, also commonly referred to as "Optical Microscopy" (Optical Microscopy or "Light Microscopy"), refers to the visible light transmitted through a sample or reflected from a sample, after passing through one or more lenses, A technology that can obtain magnified images of tiny samples. The resulting images can be directly observed with the eyes through the eyepiece, or can be recorded with a photosensitive plate or a digital image detector such as a charge-coupled device (CCD) or a complementary metal-oxide semiconductor (CMOS). It can be displayed and analyzed on a computer. ...

AI Technical Summary

Problems solved by technology

[0005] Ordinary optical microscopy using bright field illumination usually has three limitations: first, it can only image dark samples (transmission type) or strongly reflective samples (reflection type); second, the optical diffraction limit limits The highest resolution of this technology is about 200nm; the third is that the defocus information will reduce the image contrast

It can perform high-precision detection on different types of cell samples, but the system is based on the principle of cell fluorescence imaging, and cannot image other non-cellular particles and objects, so the scope of application is limited

[0010] Patent CN201710904860.1 discloses an optical coherence tomography imaging system, which uses Mach-Zehnder interference optical path, which is characterized by the use of optical fibers to simplify the system and reduce costs, but compared with the optical path structure of the F-P cavity, it is still relatively complex

Images