Gas chromatography-mass spectrometry method for determining content of 2-acetyl-1-pyrroline in fragrant rice leaves by external standard multipoint process

Abstract

The invention relates to the field of food detection, and discloses a gas chromatography-mass spectrometry method for determining the content of 2-acetyl-1-pyrroline in fragrant rice leaves by an external standard multi-point process. The method comprises: S1: sampling; S2: leaf pretreatment and sample weighing; S3: solid-phase microextraction pretreatment; S4: gas chromatography-mass spectrometryanalysis; S5: establishment of a 2-acetyl-1-pyrroline external standard multi-point process; S6: establishment of a relative recovery factor RRF; and S7: formula calculation. The data repeatability standard deviation (referring to a same extraction head) of the obtained sample is smaller than 1.5%, the reproducibility standard deviation is smaller than 8.5%, the sample detection concentration is0.0035mg / kg, and the quantitative detection limit (S / N = 10) is 0.001mg / Kg. The method provided by the invention is simple, convenient, quick, accurate, low in cost and efficient, and has good application prospects in fragrant rice quality early identification, fragrant gene genetic research and breeding early generation screening.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a gas-phase mass spectrometry method for measuring the content of 2-acetyl-1-pyrroline in fragrant rice leaves by an external standard multi-point method. Background technique [0002] Fragrant rice is deeply loved by consumers for its refreshing aroma, and the price of high-quality fragrant rice in the market is much higher than that of ordinary rice. There are many varieties of fragrant rice in China, and there are more than 200 kinds of aroma volatiles in fragrant rice, among which 2-acetyl-1-pyrroline (2AP) is the main aroma characteristic volatile (Lin et al., 1990; Ahmed et al., 1995; Tanchotikul and sieh, 1991; Maraval et al, 2008; Mathure et al, 2014), with a popcorn scent. [0003] Rice odor analysis has been carried out at home and abroad for more than 20 years. Most of the literature on the collection of rice odor uses simultaneous distillation-extraction and adsorbent tra...

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Problems solved by technology

[0003] Rice odor analysis has been carried out at home and abroad for more than 20 years. Most of the literature on the collection of rice odor uses simultaneous distillation-extraction and adsorbent trapping methods. Disadvantages such as more interference

Recently, manual solid-phase microextraction technology has also been used. Although it directly extracts the target from the sample matrix, it integrates extraction, separation, concentration, and sample injection. It does not consume organic reagents and is easy to operate. However, due to the need to collect Then transfer to gas phase injection, there is a risk of target loss during the transfer, thereby reducing the concentration of sample characteristics

For example, domestic Ying Xinghua et al. (Journal of Analytical Testing, 2011, 27, 69-71) reported that after manual solid phase microextraction was transferred to the gas phase inlet, losses were likely to occur during the transfer process; and the amount of sample used was 8 g, The extraction time is 0.2 h, the sampling volume is large and it is easy to cause insufficient extraction, etc.

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