Method for detecting content of one or more cannabinoids in industrial hemp extract by using HPLC

A technology of industrial hemp and cannabinoids, applied in the field of detection, to achieve accurate and stable detection results and simple experimental operations

Inactive Publication Date: 2020-05-08
YUNNAN LVXIN BIOLOGICAL PHARMA CO LTD
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AI Technical Summary

Problems solved by technology

Monitoring the content of various cannabinoids during the extraction process of industrial hemp is very important for product quality control, but there are no relevant literature reports on the detection method of cannabinoids in CBD oil or industrial hemp full-spectrum (broad-spectrum) oil. A set of efficient, accurate and suitable methods for the content of various cannabinoids in the intermediate products and final products of the industrial hemp extraction process is very necessary

Method used

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  • Method for detecting content of one or more cannabinoids in industrial hemp extract by using HPLC
  • Method for detecting content of one or more cannabinoids in industrial hemp extract by using HPLC
  • Method for detecting content of one or more cannabinoids in industrial hemp extract by using HPLC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example Detection of industrial hemp full-spectrum (broad-spectrum) oil

[0033] Industrial Hemp Full Spectrum (Broad Spectrum) Oil Extraction Steps:

[0034] ① After the flowers and leaves of industrial hemp are dried and crushed, they are extracted with supercritical carbon dioxide to obtain a crude extract; the extraction temperature is 50°C, the pressure of the extraction kettle is 20MPa, the extraction time is 2 hours, the temperature of the analysis kettle is 40°C, and the pressure of the analysis is 10MPa.

[0035] ②Separation and purification: Add 3 times the amount of n-hexane to dissolve the crude extract, and mix it into 200 mesh silica gel according to the material ratio of 2:1. After drying, the sample was loaded by dry method, and the column was filled with 200 mesh silica gel, eluted with n-hexane and ethyl acetate (8:1), and the mobile phase containing cannabinoids was collected. Concentrate and remove the solvent under reduced pressure in vacuo to obt...

experiment example

[0051] Experiment example Repeat experiment

[0052] Pure CBD oil samples were prepared and processed according to the methods in the examples. Take the same sample to make 6 parallel samples, operate in parallel, repeat for three days, and calculate the relative standard deviation between the parallel determination results. The results are shown in Table 2. The intra-day RSD and the inter-day RSD of the peak area of ​​the target CBD are both less than 2%, which can meet the requirement of repeatability.

[0053] Table 2 Repeatability experiment results

[0054]

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Abstract

The invention relates to the technical field of detection, in particular to a method for detecting the content of one or more cannabinoids in an industrial hemp extract by utilizing HPLC. According tothe method, the cannabinoid substance in the industrial hemp extract is ultrasonically extracted by using methanol, and is detected by using a high performance liquid chromatograph (HPLC). And the content of one or more cannabinoids in the industrial hemp extract is quantitatively obtained by adopting a retention time qualitative method and an external standard method. The method is simple to operate and good in stability, so that various cannabinoid substances in the industrial hemp extract can be quickly and accurately quantified.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for detecting the content of one or several cannabinoids in industrial hemp extract by using HPLC. Background technique [0002] Industrial hemp (Hemp) refers to cannabis with a THC (tetrahydrocannabinol) content of less than 0.3%. Nowadays, industrial hemp is becoming a global hotspot. The main active ingredients in industrial hemp are cannabinoids, including cannabinol (CBN), tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabidiol (CBDV), which are mainly used for anti-epileptic , anti-oxidation, anti-tumor, etc. [0003] The ingredient with the highest medical value in industrial hemp is CBD. Not only does CBD have no hallucinogenic effect, but it can reduce or counteract the hallucinogenic and addictive properties of THC, another chemical component in marijuana. It is effective in alleviating inflammation, pain, anxiety, and mental illness. , epilepsy and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N2030/027G01N2030/062
Inventor 刘胜贵王钲霖马海悦付彬彬李智高孔令羽
Owner YUNNAN LVXIN BIOLOGICAL PHARMA CO LTD
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