Broad-spectrum low-potential electrochemical luminescence system and construction method thereof

A construction method, electrochemical technology, applied in the field of analysis, to achieve the effect of facilitating detection, excellent water solubility, and avoiding interference

Active Publication Date: 2020-05-12
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the luminescent substance is tetraphenylporphyrin which is insoluble in water, the ECL radiation process involves an organic phase solution, and this method is difficult to be popularized in the field of aqueous phase system or ECL biochemical analysis.

Method used

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  • Broad-spectrum low-potential electrochemical luminescence system and construction method thereof
  • Broad-spectrum low-potential electrochemical luminescence system and construction method thereof
  • Broad-spectrum low-potential electrochemical luminescence system and construction method thereof

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Effect test

Embodiment 1

[0078] Add 5 mL of 80 mM ATT dropwise to 7 mL of AuCl 3• HCl (10 mg / mL), adjust pH equal to 8.0. After purification with isopropanol, water was added to adjust the concentration to 8 mg / mL. Add 12 mL of ARG (160 mmol / L) solution to 12 mL of the above solution, incubate at 37° C. for 12 hours, purify the obtained quantum dots with isopropanol, and store them at low temperature until use. Prepare a 1mg / mL solution to detect the fluorescence spectrum, such as figure 1 shown. Depend on figure 1 It can be seen that the position of the fluorescence spectrum of the quantum dot is in the band of the green light region.

Embodiment 2

[0080] Prepare 0.80mL 0.20mol / L CdCl 2 Solution, add 72.5mg HMP and 34.6μL MPA solution, dilute to 50mL. Adjust the pH to 8.0 with sodium hydroxide solution. Na 2 SeO 3 (0.8mL, 0.02M) was added dropwise into the above solution, heated for 10 minutes, then added 3.7mL of hydrazine hydrate, and refluxed for 20 hours. The obtained quantum dots were purified with isopropanol and stored at low temperature until use. The fluorescence spectrum of the quantum dots is as follows figure 2 shown. Depend on figure 2 It can be seen that the location is in the green light band.

Embodiment 3

[0082] Ru(bpy) to be purchased 3 2+ Prepare a 1mg / mL solution to detect the fluorescence spectrum, such as image 3 shown. Depend on image 3 It can be seen that the fluorescence spectrum of the quantum dot is located in the yellow-red band region.

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Abstract

The invention belongs to the field of analysis technical methods, and relates to a broad-spectrum low-potential electrochemical luminescence system and a construction method thereof. Low-toxicity carbohydrazide is used as a co-reactant, ruthenium bipyridine in a molecular form and a series of other substances in a nano-form, such as CdTe CdSe, Au and CuInS2 / ZnS quantum dots are immobilized on thesurface of a working electrode by using nafion, and anode electrochemical luminescence radiation close to 0.55 V is obtained in a water-phase buffer solution system close to physiological conditions.The luminescence potential of the system of the invention is obviously lower than the luminescence potential (1.2 V) of a classical ruthenium bipyridine / tripropylamine system, electrochemical interference can be effectively reduced, and the tolerance of an electrode material is improved.

Description

technical field [0001] The invention belongs to the field of analytical techniques and methods, and relates to a method for constructing a broad-spectrum low-potential electrochemiluminescence system. Background technique [0002] Electrochemiluminescence (electrogenerated chemiluminescence, ECL) is an analytical technique that has been widely used in the fields of biochemical analysis and clinical diagnosis. For example, Roche based on the classic ruthenium bipyridine / n-tripropylamine (Ru(bpy) 3 2+ / TPrA) system developed electrochemiluminescence kits have been used in the clinical diagnosis of more than 80 items such as tumor markers and hormones. Conventional ECL analysis requires electrochemical reactions in the coexistence of luminescent substances and co-reactants (Chem.Rev.2008, 108, 2506), Ru(bpy) 3 2+ The ECL luminescence potential of the / TPrA system is as high as 1.1-1.2V (Chem. Rev. 2004, 104(6), 3003), which requires not only good high potential tolerance of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/07C09K11/58C09K11/88C09K11/62C09K11/06G01N21/76
CPCC09K11/06C09K11/07C09K11/584C09K11/623C09K11/883C09K2211/185G01N21/76
Inventor 邹桂征傅莉
Owner SHANDONG UNIV
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