Method for identifying southern blight disease resistance of peppers

A technology for disease resistance identification and white silkworm disease, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc. The identification of disease resistance is difficult and random, so as to achieve the effects of accurate and reliable identification results, good stability of results, and high sensitivity of disease discrimination.

Pending Publication Date: 2020-05-12
HUNAN VEGETABLE RES INST
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AI Technical Summary

Problems solved by technology

[0003] At present, there is no systematic research and report on the identification of pepper silkworm and its disease resistance, and the lack of corresponding industry standards and local standards makes it extremely difficult to identify the disease resistance of white silkworm, and the progress of cultivating disease-resistant varieties lags behind. on the speed of disease occurrence and development
In production, the identification of resistance to pepper white silk disease is mainly based on field identification. The main method is to plant different pepper varieties in a certain range of fields, and use visual inspection to investigate the incidence rate during the production process. This method is affected by environmental conditions. and climatic conditions, inaccurate, imprecise, and random, not suitable as a technical standard for resistance to white silkworm of pepper

Method used

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  • Method for identifying southern blight disease resistance of peppers
  • Method for identifying southern blight disease resistance of peppers
  • Method for identifying southern blight disease resistance of peppers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The isolation and identification of embodiment 1 pathogenic bacteria

[0037] Pepper white silkworm strain (Sclerotium rolfsii sacc.) was isolated and purified from the diseased plants with typical white silkworm symptoms collected from the pepper planting base in the scientific research demonstration garden of the Hunan Vegetable Research Institute in Changsha City, Hunan Province. The symptoms are : The base of the stem grows white silk-like hyphae, spreading radially. When the humidity is high, the ground and the base of the stem are more obvious. In the late stage, the base of the stem produces a large number of small brown rapeseed-like sclerotias.

[0038] The purification process is as follows: pick out mycelia (Hypha, H) from the collected diseased strains with sterile tweezers and place them in the middle of the PDA medium, and simultaneously pick out the sclerotium (Sclerotium, S) and put them in the middle of the PDA medium. Prepare 5 dishes each of silk and ...

Embodiment 2

[0042] Example 2 Identification of Pepper Blight Disease Resistance

[0043] (1) Activating and cultivating the white silkworm of capsicum, preparing pathogenic bacteria inoculated bacterial strips

[0044]1. Preparation of PDA medium: Peel and weigh 200g of fresh potatoes, chop them, add 1L of distilled water, boil them in a pot, filter with 2 layers of gauze, remove the filter residue, add 20g of glucose to the filtrate, and distill to volume to 1L. Prepare five 200mL triangular flasks, add 5g of agar powder to each bottle, and then pour the potato juice containing glucose into the triangular flasks. Put it into a sterilizing pot after being packaged with a parafilm, and sterilize with high-pressure steam for 30 minutes at 121°C. After sterilization, when the temperature of the culture medium is 60-80°C, shake it well, and pour it into a flat plate on a sterile operating table.

[0045] 2. The pathogenic bacteria isolated in Example 1 is preserved for subsequent use. When...

experiment example

[0074] The indoor inoculation identification of disease resistance was carried out on 25 breeding materials and 10 pepper varieties by the identification method in Example 2. The identification results are shown in the table below. The results show that among the 35 materials (variety) identified, no immune materials have been found, 1 high-resistant material, 13 disease-resistant materials, 15 medium-resistant materials, 4 susceptible materials, 2 A high-sensitivity material. The material (variety) identified in this experimental example is more consistent with the disease incidence rate planted in the disease nursery and in the field, indicating that this identification method is in a controlled environment, has high inoculation efficiency, and the identification results are accurate and reliable. Breeding for disease resistance.

[0075]

[0076]

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Abstract

The invention belongs to the technical field of crop disease resistance identification, and particularly relates to a method for identifying southern blight disease resistance of peppers. The method comprises the following steps: (1) activating and culturing pepper southern blight bacteria, and preparing pathogenic bacterium inoculation strips; (2) sowing seeds in a sterilization matrix, culturingpepper seedlings for inoculation, and carrying out inoculation through a bacterium strip embedding method, wherein the inoculation parts of the pepper seedlings are 1 cm away from the base parts of pepper stems; and (3) carrying out daily management after inoculation, recording the disease level of each plant 6 days later, calculating the disease index, and evaluating the disease resistance of pepper varieties to pepper southern blight. The method for identifying the southern blight disease resistance of the peppers is provided for the first time, the inoculation method is simple and convenient to operate, the identification result is accurate and reliable, the disease judgment sensitivity is high, the method is not easily affected by environment and climate factors, morbidity is uniformafter inoculation, result stability is good, the operability is high, and the screening efficiency of disease-resistant materials can be improved favorably.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to a method for identifying disease resistance of pepper white silkworm. Background technique [0002] Pepper canker sores is a soil-borne disease that occurs during the production of peppers. The pathogen is Sclerotium rolfsii sacc., and its symptoms are: white silk-like hyphae grow on the base of the stem, spreading radially, humidity When it is large, it is more obvious on the ground and at the base of the stem. In the late stage of occurrence, a large number of small brown rapeseed-like sclerotias are produced at the base of the stem. In recent years, due to the combined effects of abnormal climate change, continuous cropping, and the degradation of main planting varieties, white silkworm of pepper has become a major epidemic disease in the production process of pepper in the middle and lower reaches of the Yangtze River, causing major ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18
Inventor 郑井元周书栋朱春晖马艳青李雪峰
Owner HUNAN VEGETABLE RES INST
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