Stirrer, cell culture tank and culture method

Abstract

The invention relates to a stirrer for a cell culture tank, the cell culture tank and a cell culture method. The stirrer comprises a middle shaft and at least blades which are in rotational symmetry by taking the axial line of the middle shaft and are connected with the middle shaft; each blade comprises a blade body, wherein the blade body spirally extends, the axial length of the blade body occupies 20-35% of the length of the middle shaft, a rotation angle of the blade body is 15-50 degrees, a maximum radial length is 20-54 mm, and a radial length is gradually reduced from the bottom to thetop. The above stirrer, culture tank and cell culture method at least have the advantages of small stirring and shearing force, a high cell yield and the like.

Description

technical field [0001] The invention relates to a stirrer, a cell culture tank and a culture method. Background technique [0002] The principle of cellular immunotherapy is to collect the body's own immune cells, culture them in vitro to increase their number by thousands of times, enhance their targeted killing function, and then reinfuse them back into the human body to kill pathogens and cancer cells in blood and tissues. cell. [0003] For the current general cancer treatment, there is no selectivity in killing tumor cells during the treatment process. While killing tumor cells, a large number of normal cells are also killed. Therefore, targeted therapy has been a hot research topic in cancer treatment. [0004] The method of cellular immunity, such as CAR-T (Chimeric Antigen Receptor T-Cell Immunotherapy, Chimeric Antigen Receptor T-cell Immunotherapy), is a treatment method that obtains T lymphocytes carrying tumor antigen-specific receptors through genetic modifica...

AI Technical Summary

Problems solved by technology

The inventors have found that the use of the above-mentioned agitator has many parts, which leads to more complicated assembly and maintenance, and whether the blades are placed obliquely or vertically, the use of the flat-shaped blades of the above-mentioned agitator will lead to relatively high cell shear stress in T cell culture. large enough to affect cell yield

[0008] In the prior art, there are also cell culture tanks without stirring function, such as the cell expansion device with the publication number CN107267389A Chinese patent application document, which adopts the method of moving the piston body to discharge the culture solution and cells, but the inventor found that, If this device is used, because there is no agitation inside the expansion device, in order to prevent cell sedimentation, the operation of centrifugation-draining of the culture medium must be performed frequently, which will consume a lot of time and materials. In the way of pushing, cells will be under great pressure in some edge areas, resulting in cell destruction, or sticking to the edge of the culture tank, reducing the yield of cells

[0009] The yield in the large-scale cultivation process of the industrial scale is lower than that of the laboratory scale. The main reason is that common industrial homogeneous mixing equipment, such as a propeller-type agitator, is used to stir and mix the culture solution and T Cells will generate vortices when they are stirred, and the shear stress of the vortices will break up T cells, resulting in low cell yields

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