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Apparatus for in-situ characterization of direct interspecies electron transfer

An electron transfer and characterization technology, applied in the field of microorganisms, can solve the problems that hinder the research process and cannot realize the in-situ monitoring of DIET biological communities, and achieve the effect of high flexibility, simple structure and high durability

Inactive Publication Date: 2020-05-19
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the microbial communities that carry out this electron transfer mechanism tend to form globules, traditional characterization methods are carried out through destructive sampling, which makes it impossible to realize the in-situ monitoring of the dynamic change process of DIET biological communities, which seriously hinders research process

Method used

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  • Apparatus for in-situ characterization of direct interspecies electron transfer
  • Apparatus for in-situ characterization of direct interspecies electron transfer
  • Apparatus for in-situ characterization of direct interspecies electron transfer

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Experimental program
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Embodiment 1

[0021] Such as figure 1 As shown, the present invention provides a device for in situ characterization of direct electron transfer between species, comprising a three-electrode anaerobic reactor and a conductive substrate. The bottom of the three-electrode reactor is hollowed out, and the conductive substrate seals the hollowed out. The area of ​​the conductive surface of the conductive substrate is larger than the area of ​​the hollow, so as to connect an external circuit connected to the conductive substrate. The conductive substrate is formed by etching gold interdigital electrodes on the surface of quartz glass. The conductive substrate is transparent and the surface is plated with a nano-gold coating. The transparency is to facilitate observation of the growth of microorganisms. The nano-gold coating can be used as an electrode.

[0022] The three electrodes of the three-electrode anaerobic reactor are respectively a salt bridge, a graphite plate and the conductive substrate...

Embodiment 2

[0024] Example 2 Characterization of the electrochemical performance of DIET biofilm:

[0025] (1) According to Example 1 ( figure 1 (Shown) made into a device;

[0026] (2) In step (1), inject the NBEF medium with a pH of 7.0 into the device, and inoculate the DIET biological community, and put it in the incubator for constant temperature cultivation at 30°C;

[0027] (3) When the substrate concentration in the system inoculated with GS15&PCA co-culture in step (2) no longer changes significantly and a thick pink biofilm is formed at the bottom (6-10 days), it is determined that the biofilm is mature;

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Abstract

The invention provides an apparatus for in-situ characterization of direct interspecies electron transfer. The apparatus comprises a three-electrode reactor and a conductive substrate, wherein the bottom of the three-electrode reactor is hollowed out, the hollowing-out is sealed with the conductive substrate, an area of the conductive substrate is larger than an area of the hollowing-out, the inside of the three-electrode reactor is filled with a culture medium and inoculated with a DIET biological community, and the DIET biological community grows into a biofilm on the conductive substrate. The apparatus can characterize the DIET community in situ without damages.

Description

Technical field [0001] The invention relates to a device for in situ characterization of direct electron transfer between species, and belongs to the technical field of microorganisms. Background technique [0002] Direct interspecific electron transfer (DIET) is an important microbial mutual metabolism mechanism, which is considered as an alternative mechanism for interspecific hydrogen transfer, and it is dominant in a variety of anaerobic environments such as anaerobic methane-generating digesters. At the same time, DIET has been confirmed to be related to the global release and consumption of methane gas, anaerobic photosynthesis and other processes, and has a huge biogeochemical impact. Research on DIET not only has environmental significance, but the bioelectrical connection components used in its mechanism may also have high guiding value for production practice. However, because the microbial communities that carry out this electron transfer mechanism tend to form globul...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/24C12M1/00G01N27/416G01N27/48G01N21/65C12R1/01
CPCC12M23/08C12M41/46G01N27/416G01N27/48G01N21/65
Inventor 周顺桂刘星詹骥
Owner FUJIAN AGRI & FORESTRY UNIV