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Reagent and method for eliminating nucleic acid aerosol pollution in air

An aerosol and nucleic acid technology, applied in the direction of disinfectants, botany equipment and methods, biocides, etc., can solve the problems of prolonging the operation time of PCR experiments, inability to solve aerosol pollution, cumbersome operation, etc., and achieve stable and uniform removal effect. High peroxide conversion rate and hydroxyl radical content, good diffusivity effect

Pending Publication Date: 2020-05-22
BIOTEKE CORP (WUXI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is cumbersome to operate, prolongs the experimental operation time of PCR, and is not suitable for use in hospitals, laboratories and other places that require efficient and rapid detection.
[0006] CN201620478005.X provides a device based on nucleic acid amplification laboratory to eliminate aerosol pollution of instruments. This patent can realize aerosol pollution of instruments, but cannot solve aerosol pollution in the air

Method used

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  • Reagent and method for eliminating nucleic acid aerosol pollution in air
  • Reagent and method for eliminating nucleic acid aerosol pollution in air
  • Reagent and method for eliminating nucleic acid aerosol pollution in air

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Reagent formula is 0.01M CuSO 4 -EDTA complex, 0.01M H 2 o 2 and 0.1M ascorbic acid.

[0058] Prepare 1L of disinfectant: 17.612g of ascorbic acid, dissolved in 100ml of 0.3mM pH6.5 sodium phosphate buffer (NaH 2 PO 4 ), add 1.5961g CuSO 4 , 3.3621g EDTA and 1ml 30% H 2 o 2 After dissolving, dilute to 1L with distilled sterilized water.

[0059] Turn on the ultrasonic atomizer, atomize the reagents, and disinfect the positive control group 1 laboratory at a spray speed of 20ml / min for 45 minutes. After the disinfection is completed, collect the filter membrane of the laboratory.

Embodiment 2

[0061] The difference from Example 1 is that the atomization method is compression atomization.

Embodiment 3

[0063] The difference from Example 1 is that the atomization method is high-speed fan atomization.

[0064] The filter membranes recovered from the positive control group 1 and Examples 1-3 were resuspended in PBS, respectively, and the fluorescence quantification was carried out to calculate the clearance rate. The results are shown in Table 1.

[0065] Table 1 The removal effect of nucleic acid aerosol in different atomization methods

[0066]

[0067] from figure 1 Visible with table 1, reagent of the present invention forms dry mist by the selected atomization mode of the present invention, and the clearance rate of nucleic acid aerosol in the air is more than 90%, and each sampling point clearance rate RSD value is less than 10%, illustrates that this The reagent and method can effectively remove the nucleic acid aerosol in the air, and the removal effect is stable and uniform.

[0068] positive control group 2

[0069] The operation is the same as that of the posit...

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PUM

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Abstract

The invention discloses a reagent and a method for eliminating nucleic acid aerosol in air. The reagent comprises peroxide, metal ions and an antioxidant, wherein the concentration of the peroxide is0.001-0.5 M, the concentration of the metal ions is 0.001-1.0 M, and the concentration of the antioxidant is 0.05-1.0 M. The reagent is high in hydrogen peroxide conversion rate and hydroxyl radical content, can effectively remove nucleic acid aerosol in air, and is quicker, more thorough, more stable and more uniform in removal of nucleic acid aerosol. The method for removing the nucleic acid aerosol in the air comprises the steps that the reagent is atomized to form dry fog, and then disinfection treatment is conducted on laboratory space. According to the method, the removal rate of the nucleic acid aerosol reaches 90% or above, and experiment requirements can be met.

Description

technical field [0001] The invention relates to a reagent for eliminating nucleic acid aerosol pollution in the air, and also relates to a method for eliminating nucleic acid aerosol pollution in the air. Background technique [0002] Polymerase Chain Reaction (PCR) is an in vitro nucleic acid amplification technique developed in the 1980s. It has outstanding advantages such as specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, and easy automation. PCR technology is a revolutionary initiative and milestone in the field of biotechnology, especially in the field of food safety testing, which shows its irreplaceable side. The biggest feature of PCR technology is its large amplification ability and high sensitivity, but extremely small amounts of pollution can also cause false positives, among which PCR amplification product pollution is the most important and most common pollution problem in PCR reactions. Aerosol is a colloidal dispersion system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/06A01N59/20A01N59/16A01N59/00A01N43/08A01N37/16A01N47/28A01N59/08A01P1/00
CPCA01N25/06A01N37/16A01N43/08A01N47/28A01N59/00A01N59/08A01N59/16A01N59/20
Inventor 周志图
Owner BIOTEKE CORP (WUXI) CO LTD
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