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A kind of green pepper extract and its use

A technology of green pepper and extract, applied in the field of green pepper extract and its bacteriostatic agent for Gram-positive bacteria, can solve problems such as difficult to extract fat-soluble alkaloids, achieve better antibacterial effect, improve total Alkaloid ratio, effect of improving extraction rate

Active Publication Date: 2021-09-07
营山椒宝宝花椒有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when using an acidic aqueous solution for extraction, only water-soluble alkaloids are often extracted, and it is difficult to extract fat-soluble alkaloids.

Method used

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  • A kind of green pepper extract and its use
  • A kind of green pepper extract and its use
  • A kind of green pepper extract and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 100 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=6.0 with 18wt% of alkyl alcohol ether glycoside AEG30, 22wt% of dipotassium hydrogen phosphate, 1.5wt% of potassium chloride and the rest of deionized water; then at 40°C Mix well, and let stand at constant temperature. According to the solid-liquid ratio of 1:12g / mL, weigh 100 mesh green pepper powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 60°C to obtain an extract. The microwave extraction conditions are: microwave power 260W; extraction time 20min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Use the polysulfone ultrafiltration membrane with a molecular weight ...

Embodiment 2

[0078] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 80 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=6.5 with 20wt% of alkyl alcohol ether glycoside AEG30, 20wt% of dipotassium hydrogen phosphate, 1wt% of potassium chloride and the rest of deionized water; then at 45°C Mix well and let stand at constant temperature. According to the solid-liquid ratio of 1:10g / mL, weigh 80 mesh green Zanthoxylum bungeanum powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 65°C to obtain an extract. The microwave extraction conditions are: microwave power 240W; extraction time 18min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Using a polysulfone ultrafiltration membrane with a molecul...

Embodiment 3

[0080] Dried green peppercorns (place of origin: Laiwu, Shandong) to moisture content ≤ 10%, and crushed to a particle size of 120 mesh to obtain green peppercorn powder. Prepare a two-phase aqueous extraction solvent, adjust pH=5.5 with 16wt% of alkyl alcohol ether glycoside AEG30, 24wt% of dipotassium hydrogen phosphate, 2wt% of potassium chloride and the rest of deionized water; then at 35°C Mix well and let stand at constant temperature. According to the solid-liquid ratio of 1:14g / mL, weigh 120 mesh green pepper powder, place it in a closed microwave extraction device, add a two-phase extraction solvent, and extract at 55°C to obtain an extract. The microwave extraction conditions are: microwave power 280W; extraction time 22min. The extract was filtered with a 0.22 μm filter membrane, added to a separatory funnel and allowed to stand for phase separation to obtain an upper phase and a lower phase. Purify the upper phase at an operating pressure of 0.2 MPa and room temp...

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Abstract

Disclosed is a green pepper extract, which is obtained from green pepper powder through extraction and purification. In addition, the use of the extract as a bacteriostatic agent is also disclosed. The total alkaloid ratio of the extract and the content ratio of antibacterial components are relatively high, the antibacterial effect on Gram-positive bacteria, especially Staphylococcus aureus is better, and it can be used as the antibacterial agent.

Description

technical field [0001] The invention belongs to the technical field of biochemical products; it relates to a green pepper extract with better antibacterial effect and its use, more specifically, it relates to a green pepper extract and its use as a bacteriostatic agent for Gram-positive bacteria . Background technique [0002] Green Zanthoxylum bungeanum is a plant of the genus Zanthoxylum Bungeanum Maxim, and generally said Zanthoxylum bungeanum refers to the dried fruit of this type of plant. There are about 250 species of this genus in the world, distributed in tropical and subtropical regions such as Asia, Africa, South America and Oceania. my country is one of the countries with the largest pepper planting area in the world, mainly distributed in Sichuan, Chongqing, Gansu, Shandong and Hebei provinces. [0003] The most common use of green Zanthoxylum bungeanum is as a condiment. It is a favorite spice of our people and is known as one of the eight great flavors. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/758A61P31/04
CPCA61K36/758A61K2236/331A61K2236/51A61K2236/53A61P31/04
Inventor 王汪杨华忠周静熊定伟史亮武碧先
Owner 营山椒宝宝花椒有限责任公司