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Cell signal pathway detection kit and detection method based on mass spectrum flow technology

A cell signal and pathway detection technology, applied in the field of cell biology, can solve the problems that cannot reveal the overall change trend of the sample, the change level of cell types, and the detection of signal pathway changes at the single cell level.

Pending Publication Date: 2020-05-22
浙江普罗亭健康科技有限公司
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Problems solved by technology

[0005] However, due to its limited detection parameters, traditional flow cytometry technology is greatly restricted in the detection of signaling pathway changes at the single-cell level in limited clinical tissue samples.
The current commercial signal pathway detection methods mainly include flow cytometry analysis, western blotting and other means of detection, which can only judge the change of the signal pathway from the overall level of the sample, and cannot reveal whether the change comes from the overall change trend of the sample or the sample. Altered levels within specific cell types

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  • Cell signal pathway detection kit and detection method based on mass spectrum flow technology
  • Cell signal pathway detection kit and detection method based on mass spectrum flow technology
  • Cell signal pathway detection kit and detection method based on mass spectrum flow technology

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Embodiment

[0052] A kit for detecting the phosphorylation and activation status of target signaling pathway proteins in cell samples based on mass spectrometry, including LD solution, F I solution, F II solution, B solution, W solution, P solution, D solution, BC (1- 10) Liquid. LD solution includes 194Pt, RPMI 1640 basal medium; F I solution includes fixation and permeation solution; F II solution includes formaldehyde and phosphate buffer; B solution is a blocking buffer for flow cytometry, which is characterized in that it contains Human immunoglobulin, mouse immunoglobulin, rat immunoglobulin, hamster immunoglobulin, bovine serum albumin, phosphate buffer; W solution is permeation buffer; P solution is fixation / permeation concentrate, Fixation / permeabilization diluent; solution D includes 191 / 193Ir, formaldehyde, phosphate buffer; BC(1-10) solution: includes Pd104, Pd105, Pd106, Pd108, Pd110, phosphate buffer.

[0053] After the above reagents are prepared, store them at 2-8°C.

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Abstract

The invention discloses a cell signal pathway detection kit and a detection method based on a mass spectrum flow technology. Through the kit and a matched signal pathway stimulant and a detection antibody, and by utilizing a mass spectrum flow detection technology, an intracellular signal path activation level can be specifically and effectively detected at a high throughput and a single cell level, the sample detection precision is greatly improved, and a false negative analysis result possibly existing in a traditional detection means is avoided to a great extent.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a cell signaling pathway detection kit and detection method based on mass spectrometry flow detection technology. [0002] technical background [0003] Mass flow cytometry detection technology is a technology that detects rare metal-labeled antibodies by mass spectrometer at the single-cell level, and performs multi-parameter, high-throughput qualitative analysis of each cell. Compared with traditional flow cytometry, this technology has the advantages of more detection parameters and higher signal-to-noise ratio. It is currently the most advanced single-cell flow detection technology and has been increasingly used in the fields of clinical medicine and biological research. Wide range of applications. [0004] Signaling pathway refers to the process in which cells in tissues and organs are stimulated by exogenous molecules or endogenous changes, and the stimulus signal is t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N1/30
CPCG01N15/1031G01N1/30G01N2015/1006G01N2015/1024G01N15/01
Inventor 王平吕聪林铖
Owner 浙江普罗亭健康科技有限公司
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