As <5 +> colorimetric detection method based on iron alkoxide nano-enzyme

A nano-enzyme and detection method technology, applied in the field of analytical chemistry, can solve the problems of limited application scope, high cost, cumbersome steps, etc.

Active Publication Date: 2020-06-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires specialized genetic engineering and biotechnology cell transformation, the steps are cumbersome and expensive, and the application range is limited

Method used

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  • As &lt;5 +&gt; colorimetric detection method based on iron alkoxide nano-enzyme
  • As &lt;5 +&gt; colorimetric detection method based on iron alkoxide nano-enzyme
  • As &lt;5 +&gt; colorimetric detection method based on iron alkoxide nano-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of iron alkoxide nanozyme

[0037] 1.2g FeCl 3 ·6H 2 O and 5.4 g of urea were dispersed in 180 mL of ethylene glycol, mechanically stirred for 20 min, and the mixed solution was reacted at 195 °C for 30 min; the iron alkoxide was collected by centrifugation, washed three times with ethanol and deionized water, and dried at 60 °C for 24 h. Its shape is a flower-shaped sphere, such as figure 1 shown.

[0038] Take the prepared 100 μL iron alkoxide nanozyme (1mg mL -1 , dissolved in deionized water) was dispersed in 2700 μL of acetic acid-acetate buffer solution (0.2M, pH 4.0), and 100 μL of TMB solution (5 mM, dissolved in ethanol) was added to the above mixed solution and incubated for 20 min. Measure the ultraviolet absorption spectrum of the mixed solution with an ultraviolet-visible absorption spectrophotometer, record the absorbance, and the full spectrum is as follows: figure 2 shown.

[0039] The superoxide anion generated in the ESR detection...

Embodiment 2

[0045] 1. Preparation of iron alkoxide nanozyme

[0046] 1.2g FeCl 3 ·6H 2 O and 5.4 g of urea were dispersed in 90 mL of ethylene glycol, mechanically stirred for 10 min, and the mixed solution was reacted at 150 °C for 20 min; the iron alkoxide was collected by centrifugation, washed three times with ethanol and deionized water, and dried at 60 °C for 24 h.

[0047] Take the prepared 100 μL iron alkoxide nanozyme (1mg mL -1 , dissolved in deionized water) was dispersed in 2700 μL of acetic acid-acetate buffer solution (0.2M, pH 4.0). 100 μL of TMB solution (5 mM, dissolved in ethanol) was added to the above mixed solution and incubated for 20 min. Measure the ultraviolet absorption spectrum of the mixed solution with an ultraviolet-visible absorption spectrophotometer, and record the absorbance.

[0048] 2. As based on iron alkoxide nanozyme 5+ Colorimetric detection method

[0049] 100μL of IA (1mg·mL -1 , dissolved in deionized water) was dispersed in 2700 μL of ace...

Embodiment 3

[0051] 1. Preparation of iron alkoxide nanozyme

[0052] 1.2g FeCl 3 ·6H 2 O and 5.4 g of urea were dispersed in 135 mL of ethylene glycol, mechanically stirred for 15 min, and the mixed solution was reacted at 175 °C for 25 min; the iron alkoxide was collected by centrifugation, washed three times with ethanol and deionized water, and dried at 60 °C for 24 h.

[0053] Take the prepared 100 μL iron alkoxide nanozyme (1mg mL -1 , dissolved in deionized water) was dispersed in 2700 μL of acetic acid-acetate buffer solution (0.2M, pH 4.0); 100 μL of TMB solution (5 mM, dissolved in ethanol) was added to the above mixed solution and incubated for 20 min. Measure the ultraviolet absorption spectrum of the mixed solution with an ultraviolet-visible absorption spectrophotometer, and record the absorbance.

[0054] 2. As based on iron alkoxide nanozyme 5+ Colorimetric detection method

[0055] 100μL of IA (1mg·mL -1 , dissolved in deionized water) was dispersed in 2700 μL of ace...

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Abstract

The invention belongs to the technical field of analytical chemistry, relates to an As <5 +> detection method, and specially relates to an As <5 +> colorimetric detection method based on iron alkoxidenano-enzyme. The method comprises the following steps: preparing 1 mg.mL <-1 > of an iron alkoxide nano-enzyme solution from deionized water, respectively dispersing 100 [mu] L of the iron alkoxide nano-enzyme solution in 2700 [mu] L of an acetic acid-acetate buffer solution, adding 100 [mu] L of As <5 +> with different concentrations, and incubating for 0.5-5 min; respectively adding 100 [mu] Lof a 5 mM TMB ethanol solution, and incubating for 10-30 min; determining the ultraviolet absorption spectrum of the mixed solution by using an ultraviolet-visible absorption spectrophotometer, recording the absorbance at the wavelength of 652 nm, and drawing a standard working curve; repeating the steps to measure the absorbance of the As <5 +> sample to be measured; and comparing with a standardworking curve to obtain the As5 + concentration. According to the invention, As <5 +> is detected by iron alkoxide nano-enzyme colorimetry, the conditions in the detection process are mild, so convenient and rapid detection of As < 5 + > is realized, and the cost is low; the detection range is as wide as 3.33-333.33 [mu] g.L <-1 >, and the lowest limit of the World Health Organization on arsenicions is met.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and relates to As 5+ detection method, especially involving an As based on iron alkoxide nanozyme 5+ Colorimetric detection method. Background technique [0002] Due to the development of minerals and the discharge of industrial wastewater, inorganic arsenic pollution is becoming more and more serious around the world. According to the survey, there are at least 20 million people in the world living in areas seriously polluted by inorganic arsenic. In nature, pentavalent arsenate (As 5+ ) and trivalent arsenite (As 3+ ) is the most common chemical form of inorganic arsenic. Inorganic arsenic is highly toxic and carcinogenic, and seriously threatens human health. The World Health Organization (WHO) strictly stipulates that the arsenic content in drinking water should not exceed 10 μg L -1 . Therefore, the quantitative detection of inorganic arsenic is of great significance to en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N21/33
CPCG01N21/78G01N21/31G01N21/33G01N2021/786
Inventor 徐雪超牛湘衡李欣潘建明
Owner JIANGSU UNIV
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