Three-dimensional Au super particle based on pi-pi directional self-assembling, preparation thereof and application of three-dimensional gold super particle in SERS substrate
A directional self-assembly, self-assembly technology, applied in metal processing equipment, nanotechnology for materials and surface science, measurement devices, etc., can solve the limitations of SERS technology commercialization, poor SERS signal reproducibility, inconsistent signal enhancement, etc. problem, to achieve the effect of low cost, easy operation and rapid detection
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Embodiment 1
[0067] This embodiment provides a preparation method of gold superparticles.
[0068] 1. (1) The specific steps for the synthesis of gold superparticles are as follows:
[0069] ① Dissolve 8μL of 10% (w / v) HAuCl4 (2mM) solution was added to 1mL of 1mM HCl aqueous solution, followed by 2.5μL of KBr solutions of different concentrations (0, 1.6mM, 6.4mM), shaken and mixed, and recorded as solution 1.
[0070] ②Dissolve 80mg of NAAN in 1mL of 1mM HCl aqueous solution (solution concentration is 320mM), and record it as solution 2.
[0071] ③Put solutions 1 and 2 in the refrigerator at 4°C for 30 minutes; then, inject 100 μL of solution 2 into solution 1 and mix by vortexing for 30 seconds, then place the mixture in a refrigerator at 4°C for 30 minutes, and the reaction is complete Afterwards, the precipitate was collected and washed three times with water to obtain gold superparticles (Au SPs) and characterize them.
[0072] (2) Morphology determination of Au SPs
[0073] Drop ...
Embodiment 2
[0103] This embodiment provides a SERS detection method for Au SPs.
[0104] According to the synthetic method of embodiment 1 gold superparticle, preparation condition is adjusted to: Br -1 / HAuCl 4 / NAAN=1 / 1 / 16 (molar ratio), the reaction temperature is 70° C., and the reaction time is 30 minutes to obtain Au SPs. Then the Au SPs were formulated into Au SPs solution (100Au SPs / mL aqueous solution), and then 20 μl of the obtained Au SPs solution was mixed with 1 mL of 1 mmol / L 4-MBA (4-mercaptobenzoic acid) or 1 mL of 10 μmol / L RBITC (rhodamine B isothiocyanate) aqueous solution was mixed and stirred for 2 hours. The Raman molecule-modified Au SPs were collected, washed three times with water, and finally dispersed in 0.2 mL of water for storage. 10 μL of Raman molecule-modified Au SPs solution (100 Raman molecule-modified Au SPs / mL) was dropped on the silicon wafer, dried and used for SERS measurement.
[0105] To investigate the SERS sensitivity of individual Au SPs, 4-...
Embodiment 3
[0121] This example provides a method for testing the stability of Au SPs in various media.
[0122] (1) According to the synthesis method of Example 1 Au SPs, the preparation conditions are adjusted to: Br -1 / HAuCl 4 / NAAN=1 / 1 / 16 (molar ratio), the reaction temperature is 70° C., and the reaction time is 30 minutes to obtain Au SPs. Then Au SPs were incubated with phosphate buffered saline solution (PBS) at pH 7.4 or Dulbecco's Modified Eagle's Medium (DMEM) medium (the concentration of Au SPs was about 100 / mL solution) containing fetal bovine serum (FBS) for 1 moon.
[0123] After incubating Au SPs with PBS or FBS for 1 month, the shape of Au SPs did not change as confirmed by its SEM images. The results showed that Au SPs had excellent stability in phosphate buffered saline (PBS) or fetal bovine serum (FBS) medium at pH 7.4.
[0124] (2) The 4-MBA-modified Au SPs prepared in Example 2 (about 100 Au SPs / mL) were incubated with DMEM medium containing fetal bovine serum (...
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