High-throughput mass spectrometry detection kit and quality inspection method thereof

Abstract

The invention discloses a high-throughput mass spectrometry detection kit and a quality inspection method thereof. The high-throughput mass spectrometry detection kit comprises a pore plate, a biological sample pretreatment material, a mass spectrometry test reagent and a quality control sample; the biological sample pretreatment material is 0.03-0.05 mg of porous silicon particles; the mass spectrum test reagent comprises an incubation solution, a cleaning solution, a matrix dissolving solution and a matrix; according to the quality inspection method, the average distance DL value between thedetection sample and the quality inspection standard database is calculated, and the two-dimensional measurement is carried out on the mass spectrum by combining the cityblock distance and the minkowski distance, so that the identification accuracy can be improved; the identification Youden index of various variants is higher than 98%; according to the quality inspection method, the quality inspection qualification rate is judged by evaluating the similarity of the mass spectra, the whole production process of the kit can be effectively controlled, and the production process stability and theproduct stability are evaluated; therefore, the high repeatability of the mass spectrum reagent in the biological sample detection process is achieved, and the variable coefficient is controlled within 15%.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection of biological samples, in particular to a high-throughput mass spectrometry detection kit and a quality inspection method thereof. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is one of the main methods used to analyze complex biological samples at this stage, and can simultaneously detect dozens or even hundreds of biomarkers in a single analysis. Compared with traditional diagnostic techniques, MALDI MS has the advantages of high sensitivity, high throughput and low cost, and is a potential clinical testing platform. Due to the lack of LC separation process, MALDI MS has high requirements for the pretreatment of complex samples. The complex sample pretreatment process will lead to the loss and missed detection of low-abundance components, and the existing technical solutions have low throughput and high cost. For example, whil...

AI Technical Summary

Problems solved by technology

[0005] In the MALDI MS test, the existing sample mass spectrometry detection reagents require complex sample pretreatment process, resulting in large batch-to-batch differences and unstable data, which greatly interferes with the discovery of biomarkers

In addition, the market also lacks effective quality control methods for mass spectrometry reagents in order to monitor the production process and product quality of mass spectrometry reagents, which cannot meet the stability requirements of industrialization and clinical testing; cosine, etc. With a single measurement, the recognition rate of various spectral variations is very low, the accuracy rate is less than 70%, and the quality of mass spectrometry peaks cannot be accurately evaluated; the market needs a reagent with simple detection steps, high throughput, specificity, and high sensitivity box and quality inspection method, the present invention solves such problems

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