Zinc ion complex peptide and complex and application thereof
A technology of zinc ions and complexes, which is applied in the field of physical and chemical engineering, and can solve the problems of lack and lack of organic zinc supplement products, etc.
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Embodiment 1
[0046] This example provides a kind of preparation method of zinc ion complex, and the steps comprise:
[0047] (1) Mix soybean protein powder with pure water at a ratio of 1:50, adjust the temperature to 50°C and pH to 8.0, and add ALcalase enzyme for enzymatic hydrolysis;
[0048] (2) Take the enzymatic hydrolysis product obtained in step (1) and inactivate the enzyme at 100°C for 5 minutes, then centrifuge, and take the supernatant to obtain the protein peptide solution.
[0049] (3) Take the protein peptide solution obtained in step (2) and perform ultrafiltration fractionation. The cutting molecular weights of the ultrafiltration membranes are 5KDa, 1K Da, and 0.5K Da respectively, and the component SPIH2 with the strongest zinc ion complexing rate of 1-5K Da is collected. (See Table 1).
[0050] (4) Get step (3) gained SPIH2 component and carry out gel chromatography separation, screen and collect the peptide component F2 (see figure 1 ).
[0051] (5) Take the peptide...
Embodiment 2
[0054] This example provides a kind of preparation method of zinc ion complex, and the steps comprise:
[0055] (1) Take soybean meal for superfine grinding or puffing and then crushing to make soybean meal protein powder, then mix soybean protein powder with pure water at a ratio of 1:10, adjust the temperature to 50°C and pH value to 7.0, and add in sequence Papain and Bacillus subtilisin for enzymatic hydrolysis.
[0056](2) Take the enzymatic hydrolysis product obtained in step (1) and inactivate the enzyme at 100°C for 5 minutes, then centrifuge, and take the supernatant to obtain the protein peptide solution.
[0057] (3) Take the protein peptide solution obtained in step (2) and perform ultrafiltration fractionation. The cutting molecular weights of the ultrafiltration membranes are 5KDa, 1K Da, and 0.5K Da respectively, and the component SPIH2 with the strongest zinc ion complexing rate of 1-5K Da is collected. (See Table 1).
[0058] (4) Get step (3) gained SPIH2 co...
Embodiment 3
[0062] This example provides a kind of preparation method and application of zinc ion complex, and the steps include:
[0063] (1) According to the amino acid composition and sequence of the zinc ion complex peptide, the zinc ion complex peptide (Lys-Tyr-Lys-Arg-Gln-Arg-Trp) with a purity of more than 95% is prepared by solid-phase peptide synthesis (see figure 2 ).
[0064] (2) Prepare zinc ion-coordinating peptide (100μM), dissolve in ZnCl-containing 2 (250 μM) in 50 mM phosphate buffer (pH 7.2), and the mixture was stirred and equilibrated at room temperature for 1 h. Use a semi-permeable membrane with a cut-off molecular weight of 500Da to remove unbound zinc by dialysis for 5 hours, collect the retentate (complex), and freeze-dry to obtain the peptide-zinc complex powder.
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