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Algicidal bacteria, screening method and application

A technology of algae-dissolving bacteria and algae-dissolving technology, which is applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., and can solve the problems of failure to rule out the influence of culture medium, ecological adaptability and application value of algae-dissolving bacteria. Considering other issues, to achieve the effect of simple and feasible method, high safety and good ecological adaptability

Inactive Publication Date: 2020-06-12
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method of isolating and screening alginolytic bacteria is mainly to infect algal cells with bacterial culture solution, and to examine the algalytic rate by measuring the concentration of chlorophyll a (Chl-a), but the ecological adaptability and application value of the algicidal bacteria screened by this method To be considered, and the influence of the medium on the growth of algal cells cannot be ruled out

Method used

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  • Algicidal bacteria, screening method and application
  • Algicidal bacteria, screening method and application
  • Algicidal bacteria, screening method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0048] In order to investigate the effect of different ages of bacteria on the algicidal effect of XXG strain, 5.6 mL of XXG bacterial suspension in lag phase, logarithmic phase, and stable phase were respectively inoculated into aeruginosa microbes containing 100 mL of Chl-a with a concentration of 1294.22 μg / L. The algae liquid was shaken in the Erlenmeyer flask for 6 days, and the algae lysis rates were 40.28%, 50.13%, and 47.19% after 6 days. Thus it can be seen that the algicidal effect of the bacterial strain in the logarithmic phase is better than that of the bacterial strains in other phases (see image 3 ).

Embodiment 2

[0050] In order to investigate the effect of different concentrations of Microcystis aeruginosa liquid on the algicidal effect of XXG strain, 5.6mL of XXG bacterial suspension in the logarithmic growth phase were inoculated in 100mL Chl-a concentration of 659.68μg / L, 1294.22μg / L, respectively. L and 4259.88μg / L Microcystis aeruginosa liquid were shaken in Erlenmeyer flasks for 6 days, and after 6 days, the algae lysis rates were 77.1%, 50.13%, and 18.60%, respectively, after converting the Chl-a concentration in the Microcystis aeruginosa liquid Respectively decreased 508.61μg / L, 648.79μg / L, 792.33μg / L (see Figure 4 ). It can be seen that the lytic ability of XXG strains to Microcystis aeruginosa increases with the increase of the number of Microcystis aeruginosa.

Embodiment 3

[0052] In order to investigate the effect of different volume ratios of bacteria and algae on the algicidal effect of the XXG strain, the XXG bacterial suspension in the logarithmic growth phase was inoculated into the Erlenmeyer flask containing 100 mL Chl-a concentration of 1294.22 μg / L Microcystis aeruginosa liquid Medium shaking culture for 6 days, the volume ratios of bacteria and algae were 5.0%, 5.2%, 5.4%, 5.6%, 5.8%, 6.0%, and the algae dissolution rates after 6 days were 36.03%, 41.66%, 42.24%, 50.13%, 44.56%. , 47.09% (see Figure 5 ).

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Abstract

The invention discloses algicidal bacteria, a screening method and application. The strain of the Paenibacillus sp. XXG is currently preserved in the China General Microbiological Culture Collection Center (CGMCC) of the Institute of Microbiology, Chinese Academy of Sciences, and the preservation number of the strain is CGMCC NO. The preservation time is April 1, 2019, and the preservation numberis CGMCC (China General Microbiological Culture Collection Center) No. 17503. The algicidal bacteria are screened from soil, and the application of the algicidal bacteria in removing microcystis aeruginosa in a water system is provided. The screened algicidal bacteria XXG have the advantages of good ecological adaptability, high safety, low extraction cost, simple and feasible method and the like,are suitable for natural water system conditions, and can effectively control the outbreak of blue-green algae.

Description

technical field [0001] The invention belongs to the field of microbiology of algae control, and in particular relates to an algae-dissolving bacterium, a screening method and application. Background technique [0002] Every summer and autumn, water blooms occur frequently in Taihu Lake, Chaohu Lake, Dianchi Lake and other important river basins in China. Hazardous algae blooms (HAB) seriously affect fishery production, residential water safety and regional ecological security. Microcystis aeruginosa is one of the dominant algal species of HAB in the Taihu Lake Basin. Because the algal toxin produced is highly toxic, difficult to degrade, and can be bioaccumulated, it has become a public hazard in the water environment. Later, in practice, it was found that during the outbreak of HAB in the Taihu Lake system, local farmers used algae water containing HAB to irrigate the paddy fields, but no cyanobacteria appeared in the water of the paddy fields. Therefore, it is feasible to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C02F3/34C12R1/01
CPCC02F3/34C02F2103/007C12N1/20C12Q1/04C12N1/205C12R2001/01
Inventor 王美娟张文艺张小梅薛静静许明宸
Owner CHANGZHOU UNIV
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