Photonic crystal sensor and method for rapidly detecting pesticide residues in fruits and vegetables by using sensor

Abstract

The invention belongs to the technical field of pesticide detection, and particularly relates to a photonic crystal sensor and a method for rapidly detecting pesticide residues in fruits and vegetables by using the sensor. The sensor is self-assembled by a vertical deposition method to obtain a template taking SiO2 microspheres as elements, a surface of the template is coated with a molecularly imprinted prepolymer solution containing a pesticide to be detected, a polymerization reaction is performed in a water bath, the template is soaked in a hydrofluoric acid after the reaction to separateSiO2 in the template, and then elution is performed to obtain the photonic crystal sensor. Compared with a traditional rapid detection method, the method can be used for qualitatively and quantitatively detecting a single pesticide, and emergency screening and detection of the target pesticide are realized; in addition, the method is not affected by fruit and vegetable substrates and a temperature, and false negative missed judgment or false positive misjudgment does not exist. The invention provides an accurate, sensitive and rapid new method for detecting the pesticides in fruits and vegetables.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, and in particular relates to a photonic crystal sensor and a method for quickly detecting pesticide residues in fruits and vegetables using the sensor. Background technique [0002] Today, the detection and analysis of pesticide residues is developing towards multi-residue and rapid analysis. At present, the commonly used rapid detection methods for pesticide residues mostly use the enzyme inhibition method, which has the advantages of simple pretreatment and fast analysis speed, but this type of technology can only detect organophosphorus and carbamate pesticides, and can only measure the total amount of pesticides The qualitative and quantitative detection of a single pesticide cannot be carried out; the enzyme activity is easily affected by the fruit and vegetable matrix, and there are false negatives or false positives; the enzyme activity is also easily affected by the ambient te...

AI Technical Summary

Problems solved by technology

At present, the commonly used rapid detection methods for pesticide residues mostly use the enzyme inhibition method, which has the advantages of simple pretreatment and fast analysis speed, but this type of technology can only detect organophosphorus and carbamate pesticides, and can only measure the total amount of pesticides The qualitative and quantitative detection of a single pesticide cannot be performed; the enzyme activity is easily affected by the fruit and vegetable matrix, and there are false negatives or false positives; the enzyme activity is also easily affected by the ambient temperature. When the temperature is lower than 37 ° C, the enzyme reaction speed As it slows down, the reaction time should be relatively prolonged after the drug is added, and the detection time will also be extended accordingly.

[0003] In addition, although traditional pesticide residue detection methods can obtain high-sensitivity and high-accuracy test results through the detection of large-scale instruments (such as liquid chromatography, gas chromatography, chromatograph-mass spectrometry, etc.), the pretreatment process is cumbersome and has a great impact on the operation. The technical level of personnel is high, time-consuming and costly to detect, which brings inconvenience to the supervision work

Images