Hypoglycemic active ingredient in panax japonicus, hypoglycemic composition and application thereof

A composition and technology of ginseng saponins are applied in the field of traditional Chinese medicine, and can solve the problems of single target point, obvious toxic and side effects, etc.

Pending Publication Date: 2020-06-19
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used oral hypoglycemic drugs in China are divided into insulin secretagogues, insulin sensitizers, α-glucosidase inhibitors and high glucose damage repair agents, such as glibenclamide, metformin, acarbose and hydroxyl Calcium ben

Method used

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  • Hypoglycemic active ingredient in panax japonicus, hypoglycemic composition and application thereof
  • Hypoglycemic active ingredient in panax japonicus, hypoglycemic composition and application thereof
  • Hypoglycemic active ingredient in panax japonicus, hypoglycemic composition and application thereof

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Experimental program
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Effect test

preparation example Construction

[0110] 1.6.1 Preparation method of total saponins of Panax ginseng (one to five methods)

[0111] Method 1: Grind the dried bamboo ginseng medicinal material into coarse particles, weigh 10 kg, reflux extract with 70% ethanol for 3 times, each time for 2 hours, filter, combine the filtrates, and concentrate under reduced pressure to obtain the extract (take part of the dried part to obtain the total Extract 1) Add appropriate amount of water to suspend, filter, apply to D101 macroporous resin, start gradient elution from 10%, 30%, 50%, 70%, 90% ethanol, monitor the elution according to the thin-layer plate TLC color method Does the eluent contain bamboo ginsenoside V, pseudoginsenoside RT1, bamboo ginsenoside IV, bamboo ginsenoside IVa, any of the four appear in the eluent and start to collect until all four are eluted from the resin The collection was stopped, and this part of the eluate was concentrated and dried to obtain the total saponins of Panax ginseng 1.

[0112] Met...

Embodiment 1

[0124] 1. Effect of total saponins of Bamboo Ginseng on glucose consumption in HePG2 cell insulin resistance model [1]

[0125] When the cells grow to 80-90% confluence in the culture flask, discard the medium and wash it twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour off the trypsin, and add the medium containing 10% serum Stop the digestion, pour out the medium, add new medium containing 10% fetal bovine serum, blow the cells gently; count the cells, dilute the cells to 8×10 4 cells / mL, inoculated in a 96-well plate, inoculated 200 μL per well, and added 200 μL of LD-Hank’s solution to each well around it. Place the 96-well plate in 5% CO after seeding the cells 2 , Cultivate in a constant temperature incubator at 37°C, when the cells grow to 80-90% confluence, suck out the culture medium, and divide the cells into: (1) blank group 180 μL high-sugar DMEM and 20 μL 10% BSA in PBS solution; (2) Model group: 180 μL high-sugar DMEM and 20 μL 1 mmol / ...

Embodiment 2

[0139] 2. Effect of total saponins of Bamboo Ginseng on cell viability and NO secretion in high glucose-induced HUVEC injury model [2-6]

[0140] When the cells were incubated in the culture flask until they were 90% confluent, discard the medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour off the trypsin, and add 10% serum (w / v, g / 100ml) medium to stop digestion, discard the medium, add 4mL of new medium containing 10% fetal bovine serum (w / v), blow the cells gently; perform cell counting, and dilute the cells to 3×10 4 cells / mL, inoculated in 96-well plate, inoculated 200 μL per well.

[0141] Place the 96-well plate in 5% CO 2 1. Cultivate in a constant temperature incubator at 37°C. When the cells grow to 90% confluence, replace them with low-sugar DMEM medium containing 1% fetal bovine serum and culture for 24 hours to synchronize the cells. The cells were divided into blank control group, model control group and drug administrati...

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Abstract

The invention discloses a hypoglycemic active ingredient in panax japonicus, a hypoglycemic composition and application thereof. 1-12 compounds are separated from the panax japonicus, and that the 3 #compound and the 5-12 compounds have hypoglycemic activity is verified; the 1-12 compound composition and the 5-8 compound composition have hypoglycemic activity; and that panax japonicus total saponins and panax japonicus medicinal material extracts have hypoglycemic activity is verified. By means of in-vitro and in-vivo hypoglycemic experimental models, various hypoglycemic active ingredients in the panax japonicus, the hypoglycemic composition with the hypoglycemic advantage and related activity and application of the hypoglycemic active ingredient in the panax japonicus and the hypoglycemic composition with the hypoglycemic advantage in the aspect of diabetic renal complications are obtained through research. Hypoglycemic traditional Chinese medicine with small side effects and a goodeffect is screened out.

Description

technical field [0001] The present invention belongs to the technical field of traditional Chinese medicine, and specifically relates to the hypoglycemic active ingredient in Panax ginseng and its hypoglycemic advantageous composition and application. Background technique [0002] Diabetes is a kind of common disease, frequently-occurring disease, along with the raising of people's living standard, the sickness rate of diabetes is increasing year by year. At present, the commonly used oral hypoglycemic drugs in China are divided into insulin secretagogues, insulin sensitizers, α-glucosidase inhibitors and high glucose damage repair agents, such as glibenclamide, metformin, acarbose and hydroxyl Calcium benzenesulfonate, etc., various types of anti-diabetic drugs may have a single therapeutic target, or have obvious toxic and side effects. In recent years, it has been favored by people to find multi-target anti-diabetic drugs in traditional Chinese medicine. [0003] This st...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P3/10A61P13/12A61K36/258
CPCA61K31/704A61P3/10A61P13/12A61K36/258
Inventor 杨小林王如锋
Owner SHANGHAI UNIV OF T C M
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