11 results about "PANAX JAPONICUS ROOT" patented technology

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The invention discloses a method for preparing rare saponin by utilizing panax japonicus endogenous enzyme, and relates to the technical field of extraction, the panax japonicus plant material or crude extract thereof is directly taken as a starting point, the enzyme system is simple and convenient to obtain (fresh medicinal material low-temperature homogenate extraction or direct use of freeze-dried powder), the reaction system is simple, and the operation is easy. According to the preparation process provided by the invention, the catalytic action of endogenous enzyme is fully utilized, so that the introduction of exogenous substances (acid-base, protein and the like) in the extract is completely avoided; the method disclosed by the invention is high in catalytic efficiency, can quickly realize complete conversion of saponin (within 10min) under specific conditions, and has extremely high industrial application.

The invention discloses application of panax japonicus polysaccharide in preparation of drugs for resisting porcine reproductive and respiratory syndrome virus. According to the application disclosed by the invention, the regulation and control effect of the panax japonicus polysaccharide on the replication of the porcine reproductive and respiratory syndrome virus (PRRSV) is studied, and the fact that the panax japonicus polysaccharide can effectively inhibit the replication of the PRRSV on a Marc-145 cell is found for the first time. On the basis, the invention provides a new application of the panax japonicus polysaccharide in preparation of the medicine for resisting the porcine reproductive and respiratory syndrome virus, and preferably, the concentration of the panax japonicus polysaccharide is 12.5 mu g / mL-100 mu g / mL. The research on the antiviral activity of the panax japonicus polysaccharide is enriched, and meanwhile, a new technical means is provided for research and clinical application of anti-PRRSV drugs.

The invention provides application of a pharmaceutical composition in preparation of anti-influenza virus drugs, and belongs to the technical field of biological medicines. The invention provides a pharmaceutical composition of carabrone, panax japonicus saponin IVa and plantamajoside. Multi-target intervention is realized aiming at key stages (such as adsorption, replication and release) of an influenza virus life cycle. Experiments show that the composition can significantly inhibit replication and proliferation of influenza viruses. The natural component composition disclosed by the invention has the advantage of higher safety, provides a safer, effective and economic treatment choice for clinic, and has remarkable conversion value and market potential.

The invention provides a seedling raising method for aseptic seedlings of panax japonicus. The seedling raising method comprises the following steps: step 1, obtaining disinfected tender leaves; step 2, obtaining sterile panax japonicus leaves; step 3, transferring the sterile panax japonicus leaves obtained in the step 2 to a callus induction culture medium for culture to obtain panax japonicus leaf calluses; step 4, transferring the panax japonicus leaf calluses to a subculture medium for culture to obtain panax japonicus leaf subculture callus particles; step 5, transferring the callus to an adventitious bud culture medium for induction to obtain panax japonicus adventitious buds; step 6, transferring the adventitious buds of the panax japonicus leaves to a bud generation subculture medium for culture to obtain panax japonicus cluster buds; step 7, culturing the cluster buds on a rooting culture medium for 35 days for rooting to obtain aseptic seedlings of the panax japonicus; according to the method, callus induction, adventitious bud and rooting induction formula screening is carried out based on the panax japonicus leaf explant, and a reference basis is provided for efficient establishment of a panax japonicus aseptic seedling system.

The invention belongs to the technical field of food processing, and particularly relates to a panax japonicus and dendrobium protocorm co-extract as well as a preparation method and application thereof. The preparation method of the co-extract comprises the following steps: (1) extraction: mixing panax japonicus and dendrobium protocorm, adding a natural deep eutectic solvent, controlling the solid-to-liquid ratio to be 1: (30-50) g / mL, and extracting under an ultrasonic condition to obtain a crude extract; (2) anti-solvent precipitation and purification: dropwise adding the crude extract obtained in the step (1) into excessive ethanol, standing for precipitation, and carrying out centrifugal separation to obtain a precipitate; and (3) drying treatment: drying the precipitate obtained in the step (2) at 40 DEG C until the weight is constant, so as to obtain the panax japonicus and dendrobium protocorm co-extract. The co-extract can remarkably promote proliferation and acid production of various probiotics, effectively regulate intestinal flora balance, show excellent prebiotic activity and have the potential of being developed into functional medicines or health-care foods.

This utility model discloses a storage and preservation device for bamboo-joint ginseng, including a sealed box. A light-shielding layer is fixedly installed on the inner wall of the sealed box, and the box is divided into multiple inner cavities by at least one dividing layer. A drawer unit is slidably installed in each inner cavity, and the bottom of the drawer unit is provided with a wavy, hollowed-out elastic silicone mesh tray. The light-shielding layer is connected to the lower part of the dividing layer via a disassembly and assembly mechanism. This utility model offers upgraded physical protection: the hollowed-out structure of the wavy, elastic silicone mesh tray disperses the stress on the bamboo-joint ginseng nodes, resulting in a reduced fracture rate when the ginseng is subjected to a 500g load. The light-shielding layer is efficiently disassembled and assembled: the Y-shaped insertion groove and the inclined guide design of the limiting block shorten the replacement time of the light-shielding layer, and the elastic component automatically locks to prevent loosening.

The invention discloses a children growth promoting preparation, which belongs to the technical field of traditional Chinese medicine preparations and comprises fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger, angelica sinensis, fructus amomi, radix inulae inula, pericarpium citri reticulatae, serissa serissoide, panax japonicus, rhizoma cibotii, radix scrophulariae and cattle bone powder. The preparation integrates the traditional Chinese medicine theory of tonifying spleen and kidney and regulating qi and blood, wherein the fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger and the like can tonify spleen and promote appetite, significantly improve the digestive function and improve the absorption rate of nutrient substances; the Tibet inula root and the fructus amomi regulate middle-jiao qi movement, relieve abdominal distension and qi stagnation and further optimize the absorption state; the panax japonicus, the rhizoma cibotii and the cattle bone powder directly act on a skeletal system to tonify kidney and strengthen bone, promote cartilage cell proliferation and bone longitudinal growth, and effectively improve height and growth speed. The formula gives consideration to intervention on growth disorder problems such as precocious puberty and the like, and through two-way regulation of nourishing yin and purging fire and tonifying kidney and replenishing essence, advanced bone age is delayed, and precious time is won for height increase.

The present disclosure provides a plant composition comprising a plant extract, wherein the plant extract is present in the plant composition in an amount effective to produce a beneficial effect on intestinal health of an animal. The benefits may include one or more effects on the intestinal health of the animal, which may include one or more improvements in the intestinal health and immune health of the animal. The plant extracts can comprise dandelion leaf extracts, dandelion leaves, ground dandelion leaves, dandelion roots, panax japonicus, ferula asafetida, ligustrum lucidum, fennel, Indian mulberries, quispualis indica, capsicum annuum, Indian nelumbo nucifera, red peppers / capsicum, shiitake mushroom, custard apples, hericium erinaceus, caraway, saw palmetto, phoenix-tail cymbidium, mountain lily yucca, carob beans, wild lupin, goat heads, sweet rosa roxburghii tratt, damnacanthus indicus and herba lycopi; , Amaranthus spinosus, and Sambucus williamsii, or any combination thereof. Other aspects are also provided herein.

The invention discloses panax japonicus formula granules and a preparation method thereof, and the preparation method comprises the following steps: (1) repeatedly crushing panax japonicus into superfine powder, and then breaking walls by using a wall breaking machine; (2) adding water or an ethanol solution into a wall breaking machine, carrying out wall breaking extraction, filtering, and concentrating filtrate into extract; and (3) adding auxiliary materials into the concentrated extract to prepare granules. Compared with previous extraction or heating or reflux extraction, wall-breaking extraction is adopted, the extraction efficiency of active ingredients such as saponin and polysaccharide is high, in-vitro and in-vivo blood glucose reducing experimental models show that the prepared granules have a good blood glucose reducing effect and small side effects, a simple, convenient and rapid formula selection is provided for clinical doctors to make a prescription, and the preparation process is simple and convenient. The novel traditional Chinese medicine granule decoction piece convenient to take and carry is provided for patients, the medication compliance is enhanced, and the good social and economic effects are achieved.

This invention discloses the application of *Gynostemma pentaphyllum* polysaccharide in the preparation of drugs against porcine reproductive and respiratory syndrome virus (PRRSV). This invention explores the regulatory effect of *Gynostemma pentaphyllum* polysaccharide on PRRSV replication, and for the first time discovers that *Gynostemma pentaphyllum* polysaccharide can effectively inhibit PRRSV replication in Marc-145 cells. Based on this, this invention proposes a new application of *Gynostemma pentaphyllum* polysaccharide in the preparation of drugs against PRRSV, preferably with a concentration of 12.5 μg / mL to 100 μg / mL. This invention enriches the research on the antiviral activity of *Gynostemma pentaphyllum* polysaccharide and also provides a new technical means for the research and clinical application of anti-PRRSV drugs.