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SNP (Single nucleotide polymorphism) site for detecting O-type variation of ABO blood group system

A technology of ABO blood type and gene coding, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of affecting blood group antigens, inaccurate typing, and difficult typing

Active Publication Date: 2020-06-19
QINGDAO BLOOD CENT
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Problems solved by technology

[0005] At present, blood centers or clinical blood transfusion departments in various places use serological tests to identify ABO blood types for blood donors and patients, but serological tests will be interfered by diseases, drugs or individual conditions, such as certain diseases such as tumors and leukemia, which will affect blood group antigens As a result, the positive typing is inaccurate; and the interference of autoantibodies or irregular antibodies in the serum will interfere with the reverse typing, resulting in difficulty in typing or even wrong typing

Method used

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  • SNP (Single nucleotide polymorphism) site for detecting O-type variation of ABO blood group system
  • SNP (Single nucleotide polymorphism) site for detecting O-type variation of ABO blood group system
  • SNP (Single nucleotide polymorphism) site for detecting O-type variation of ABO blood group system

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Embodiment 1

[0024] Example 1: Screening of SNP markers

[0025] 1. Extraction of peripheral blood genomic DNA:

[0026] In compliance with the relevant national policies and regulations, and on the basis of the consent of the sampling subjects, 2-5ml of peripheral venous blood from members of the O-type variant gene family was drawn and placed in EDTA-Na 2 In anticoagulant tubes, freeze at -80°C for later use; DNA extraction uses the DNA extraction kit from LIFE Company, and the specific operation process is as follows:

[0027] After the frozen EDTA anticoagulated blood was thawed at room temperature, 500 μl was put into a centrifuge tube, and an equal volume of erythrocyte lysate (pH 8.0) was added, vortexed for 5 minutes, centrifuged at 12,000 rpm for 5 minutes, and the supernatant was discarded. Add 500ul of red blood cell lysate repeatedly, vortex and mix for 5 minutes, centrifuge at 12000rpm for 5 minutes, and discard the supernatant.

[0028] The precipitate was vortex-mixed for ...

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Abstract

The invention aims to provide an SNP (Single nucleotide polymorphism) site for detecting O-type variation of an ABO blood group system. In a coding region of a novel O-type variation gene, gtgacgctg basic groups are deleted from 484 to 492 sites from an initiation codon. The invention provides a novel O-type variation gene, so that an ABO gene bank is perfected, and a way for rapid genediagnosis, gene screening and genetic counseling is provided. An application effect shows that the SNP site and a detection primer of the gene provided by the invention can be effectively applied to rapid detection of a novel O-type variation gene mutation site in peripheral blood of blood donors and clinical patients.

Description

technical field [0001] The invention belongs to the technical field of gene diagnosis products, and in particular relates to a SNP marker for detecting O gene mutation in the ABO blood group system. [0002] Background of the invention [0003] The ABO blood group system is the first human blood group system discovered and named by Austrian pathologist and immunologist Karl Landsteiner in 1900. It is also the most important blood group system so far. It plays an important role in clinical blood transfusion and neonatal hemolytic disease , he also won the Nobel Prize in Physiology and Medicine. The ABO blood group system is a blood group system based on the presence or absence of specific antigens (agglutinogens) A and B on the surface of red blood cells. According to the distribution of agglutinogens A and B, red blood cells are divided into four types: A, B, AB and O. Further, in 1924, German scholar F. Bernstein proved that the ABO blood type is controlled by three multip...

Claims

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Application Information

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IPC IPC(8): C12Q1/6881C12Q1/6858C12N15/11
CPCC12Q1/6881C12Q1/6858C12Q2600/156C12Q2531/113C12Q2535/101
Inventor 朱于莉刘丽韩斌冯智慧胡彬
Owner QINGDAO BLOOD CENT
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