Borneol and neurotrophic factor combined composition and application thereof
A technology of neurotrophic factors and trophic factors, applied in drug combinations, active ingredients of hydroxyl compounds, drug delivery, etc., can solve the problems of unused retinal disease treatment, achieve good drug compliance, promote full dissolution, safety and convenience degree of increased effect
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Embodiment 1
[0038] Example 1 Preparation of Borneol CNTF Eye Drops
[0039] (1) Preparation of gel matrix eye drops sample: Weigh 1.0 g of carboxymethyl cellulose sodium (CMC-Na), add 50 g of water for injection, stir to make it evenly dispersed, leave it at room temperature overnight, and add the remaining 49 g of water for injection. Stir for 5-10 min, and let it stand to obtain 1% CMC-Na gel matrix for use.
[0040] (2) Weigh 1.336g of polyoxyethylene hydrogenated castor oil 40 (RH40) and 0.334g of polyethylene glycol 400 (PEG400), stir for 10min and mix well for use;
[0041] (3) Weigh 0.025 g of borneol and add it to step (2), stir to completely dissolve it;
[0042] (4) Weigh 200μg of CNTF and dissolve it with 2mL phosphate buffered saline (PBS) for use;
[0043] Add (3) and (4) to 10g of 1% CMC-Na gel, stir for 10 minutes to make it evenly dispersed, then add 86.105g of 1% CMC-Na several times, stir to make it evenly dispersed, adjust the pH The value is 6.0-8.0, that is, the concentration...
Embodiment 2
[0045] Example 2 The effect of different concentrations of borneol on CNTF retinal distribution
[0046] The experimental animals New Zealand rabbits were randomly divided into 6 groups, physiological saline group, borneol eye drops group, CNTF eye drops group, and different concentrations of borneol CNTF eye drops group (borneol concentration 0.025%, 0.05%, 0.1%), respectively. 50μL of the corresponding eye drops were instilled into the eyes, before administration (0h) and 15 minutes, 30 minutes, 1 hour, and 2 hours after administration, 5 rabbits in each group were randomly selected to be sacrificed and the retinas were obtained by enzyme-linked immunosorbent method (ELISA) method to detect the content of CNTF in retinal tissues, each sample is set with three multiple holes, and the average value is taken. The results showed that after 15 minutes of administration, the content of CNTF in the retina of the borneol group and the blank group was determined to be 2.8±0.0pg / mL, 4.3±...
Embodiment 3
[0049] Example 3 Effect of Borneol CNTF Eye Drops on Retinal Function of Rats with Retinal Degeneration
[0050] The rat model of retinal degeneration was induced by tail vein injection of 40 mg / kg sodium iodate, and the rats were randomly divided into normal saline group, borneol eye drops group, CNTF eye drops group, and borneol CNTF eye drops group. Normal SD rats were routinely reared as a control, and the drug was ordered 4 times a day, 10 μL each time, 14 days after the administration, electroretinogram (ERG) was performed to detect the retinal function. After dark adaptation, general anesthesia combined with local anesthesia, pupil dilation, animal insulation device, rat-specific circular corneal electrode, standard flash stimulation to induce retinal comprehensive potential, passband: 0.1-1~300-1000Hz single stimulation. Press retina Figure 5 Item measurement, record the value. Including scotopic ERG, maximum mixed reaction, oscillation potential (OPs), photopic condit...
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