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Collagen hydrophobic modifier, preparation method thereof and method for modifying collagen

A hydrophobic modifier and collagen technology, applied in textiles and papermaking, single-component protein rayon, fiber chemical characteristics, etc., can solve the problems of easy introduction of toxicity by organic solvents, low modification efficiency, and difficulty in control, and achieve improvement Structural stability and electrostatic spinnability, improving hydrophobicity, and reducing denaturation effects

Active Publication Date: 2020-06-23
FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current collagen hydrophobic modifiers (acid anhydride, acid chloride, fatty aldehyde, etc.) have problems such as uneven solid / liquid reaction, difficulty in control, easy introduction of toxicity by organic solvents, and low modification efficiency.

Method used

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  • Collagen hydrophobic modifier, preparation method thereof and method for modifying collagen
  • Collagen hydrophobic modifier, preparation method thereof and method for modifying collagen
  • Collagen hydrophobic modifier, preparation method thereof and method for modifying collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0035] Dissolve 0.1 part of lauric acid in 1 part of dimethylformamide (DMF) and place in a 100 mL round bottom flask. Afterwards, weigh 0.5 part of thio-NHS and 0.5 part of dicyclohexylcarbodiimide, add them in sequence to the DMF solution of lauric acid, keep stirring under closed conditions, and react at room temperature for 2 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 0.5 parts of ethyl acetate, let it stand for 48 hours, precipitate out, filter the filter cake, dry in air, and obtain lauric acid-thio NHS ester.

[0036] A method for modifying collagen, comprising the steps of:

[0037] Weigh 10 parts of fish skin collagen and dissolve in 5 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 0.5 mol / L NaOH solution to adjust the pH to 9-10. Add 0.01 part of lauric ...

Embodiment 2

[0039] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0040] 10 parts of octanoic acid was dissolved in 20 parts of dimethylformamide (DMF) and placed in a 100 mL round bottom flask. Afterwards, 20 parts of thio-NHS and 30 parts of dicyclohexylcarbodiimide were weighed, added sequentially to the DMF solution of octanoic acid, stirred under airtight conditions, and reacted at room temperature for 2 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 50 parts of ethyl acetate, let it stand for 2 hours, precipitate out, collect the filter cake by filtration, and dry in air to obtain octanoic acid-thio NHS ester.

[0041] A method for modifying collagen, comprising the steps of:

[0042] Weigh 150 parts of porcine cartilage collagen and dissolve in 50 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 5 mol / L NaOH solution to adjust t...

Embodiment 3

[0044] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0045] 5 parts of palmitic acid were dissolved in 10 parts of dimethylformamide (DMF) and placed in a 100 mL round bottom flask. Afterwards, weigh 10 parts of thio-NHS and 15 parts of dicyclohexylcarbodiimide, add them in order to the DMF solution of palmitic acid, keep stirring under closed conditions, and react at room temperature for 13 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 25 parts of ethyl acetate, let it stand for 24 hours, precipitate out, collect the filter cake by filtration, and dry in air to obtain palmitic acid-thio NHS ester.

[0046] A method for modifying collagen, comprising the steps of:

[0047]Weigh 80 parts of cowhide collagen and dissolve in 25 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 3 mol / L NaOH solution to adjust the pH to 9-10. ...

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Abstract

The invention discloses a collagen hydrophobic modifier, a preparation method thereof and a method for modifying collagen. The preparation method of the collagen hydrophobic modifier comprises the following steps: sequentially adding sulfo-NHS and dicyclohexylcarbodiimide into a fatty acid solution, uniformly stirring and mixing, reacting at room temperature for 2-24 hours, filtering after the reaction is finished, collecting filtrate, adding an organic reagent, standing for 2-48 hours, precipitating, filtering, collecting and drying a solid-phase product. The collagen hydrophobic modifier prepared by the invention can effectively improve the structural stability and hydrophobicity of collagen and electrospun collagen fibers.

Description

technical field [0001] The invention belongs to the technical field of collagen modification, and in particular relates to a collagen hydrophobic modifier, a preparation method thereof and a method for modifying collagen. Background technique [0002] Electrospinning technology is currently one of the most important methods for preparing nanofibers. Electrospun fibers have the characteristics of controllable diameter, large specific surface area, and high porosity, which can meet the requirements of tissue engineering scaffolds for fiber size and spatial structure. In recent years, collagen-based electrospun nanofiber materials have attracted great interest from researchers all over the world. However, most of the current reports on collagen electrospinning involve the phenomenon of collagen denaturation, such as the loss of collagen triple helix conformation, the decrease of collagen denaturation temperature and relative molecular weight, and the dissolution of electrospun ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D207/46C08H1/00D01F4/00D01F8/02
CPCC07D207/46C08H1/00D01F4/00D01F8/02
Inventor 刘文涛李培玉白聪张元智陈胜李国英
Owner FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
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