A strain of aquatic probiotics with lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral functions and its application

A technology of fermented products and high-fat feed, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of unfavorable growth of traditional probiotics such as lactic acid bacteria, susceptibility to disease, dependence, etc.

Active Publication Date: 2021-05-11
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, recent studies have found that traditional probiotics have disadvantages and risks in the application of fish: one is that they depend on the growth status of the strains in the fish gut, because the water environment and the relatively low temperature environment in the fish gut (<37°C) , is not conducive to the growth of traditional probiotics such as lactic acid bacteria; the second is that traditional probiotics have a "stop feeding risk" when applied to fish due to their "non-indigenous" nature. susceptible to disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of aquatic probiotics with lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral functions and its application
  • A strain of aquatic probiotics with lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral functions and its application
  • A strain of aquatic probiotics with lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral functions and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1, the acquisition and identification of Cetobacterium somerae XMX-1

[0101] 1. Isolation and purification of XMX-1 bacteria

[0102] In an anaerobic incubator, take the intestinal content of wild-type zebrafish, dilute it with PBS buffer with a concentration of 10mM and a pH value of 7.4, and culture it with ampicillin-resistant (100ug / ml) GAM solid medium for 24 hours, and obtain a single colony , named XMX-1.

[0103] 2. Identification of XMX-1

[0104] 1) Morphological identification

[0105] Observe the growth morphology of the XMX-1 isolated in the above 1 on the ampicillin-resistant (100ug / ml) GAM solid medium.

[0106] The result is as figure 1 As shown, colony size: 2-6mm; round, moist, opaque, light yellow, with irregular edges.

[0107] 2) Physiological and biochemical identification

[0108] The XMX-1 isolated in the above 1 was streak cultured on GAM solid medium, and cultured at 28°C for about 24 hours under anaerobic conditions. Under ste...

Embodiment 2

[0121] Example 2, Under the induction of high-fat feed, the research of Cetella soroi on fish with lipid-lowering, anti-inflammatory and anti-apoptotic functions

[0122] 1. Zebrafish fed with high-fat diet after transfer of Cetella soxleyi

[0123] 1. Germ-free zebrafish high-fat model

[0124] 4 days after fertilization (4dpf, day post-fertilization) 150 (each fish body length is about 4.0-4.2mm; each fish body weight is about 0.6-0.8 mg) sterile Tu strain zebrafish cultured in 500ml GZM culture medium 1. High-fat feed was fed (the amount of high-fat feed was 3-5% of fish body weight per day, 2 times a day; sterile zebrafish were fed ad libitum) for 7 days to establish a sterile zebrafish high-fat model.

[0125] 2. Cetella sordii bacteria liquid and Plasmomonas bacterial liquid

[0126] In the liquid GAM substratum, respectively cultivate the Soxleyeti bacillus XMX-1 and Plasmomonads YZ2 in the embodiment 1, cultivate 12h in the anaerobic incubator at 28 ℃, obtain the Sox...

Embodiment 3

[0163] Embodiment 3, Cetella soxleyi has antiviral function research on fish

[0164] 1. Cetella sordii XMX-1 bacterial liquid or Plasmomonads YZ2 bacterial liquid

[0165] In the GAM liquid culture medium, respectively cultivate the Soxleyeti bacillus XMX-1 and Plasmomonas YZ2 in the embodiment 1, cultivate 12h in the anaerobic incubator for 28 ℃, obtain the Soxleyeti bacillus XMX-1 bacterium liquid (10 8 CFUs / mL) and Pseudomonas YZ2 bacterial solution (10 9 CFUs / mL).

[0166] 2. Carp spring viremia virus

[0167] Using EPC cells (ATCC: CRL-2872) to conduct carp spring viremia virus (SVCV) (the virus is recorded in the following literature: Liu Liyue. Molecular mechanism and proteomics research on the autophagy induced by SVCV infection of EPC cells[ D]. Huazhong Agricultural University, 2014.) Proliferation; EPC cell culture medium is Minimum Essential Media (MEM) medium containing 10% fetal bovine serum, the culture condition is 25 ℃, 5% CO 2 ;

[0168] The specific me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an aquatic probiotic with functions of lowering lipids, inhibiting inflammation, resisting apoptosis and anti-virus and its application. The present invention provides a strain of Cetobacterium somerae XMX-1, which is an indigenous bacterium in the intestinal tract of fish, the preservation number of which is CGMCC No.18908. The present invention finds that Cetobacterium somerae XMX-1 has lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral effects; using Cetobacterium somerae XMX-1 in water with a bath concentration of 10 6 10 CFUs / mL or in feed 8 CFUs / g bacteria cultured fish can achieve lipid-lowering, anti-inflammatory, anti-apoptotic and anti-viral effects; therefore, this strain can be used as aquaculture probiotics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of aquatic probiotics with the functions of lowering lipid, inhibiting inflammation, anti-apoptosis and anti-virus and its application. Background technique [0002] Probiotics are living microorganisms that, in appropriate doses, have a beneficial effect on the health of the host. Lactic acid bacteria are the most widely used probiotic strains, mainly including Lactobacillus and Bifidobacterium. In 2013, the Ministry of Agriculture and Rural Affairs approved 30 species of microorganisms for fish feeding, including 20 species of lactic acid bacteria (including 8 species of Lactobacillus and 6 species of Bifidobacteria). The use of feed microorganisms to improve fish growth performance and improve fish immune disease resistance has been confirmed by a large number of studies. [0003] At present, the traditional probiotics used in aquatic products are not the in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18A01K61/13C12R1/01
CPCA23K10/18A23K50/80A01K61/13C12N1/20C12N1/205C12R2001/01Y02A40/81
Inventor 周志刚张震解明旭冉超杨雅麟高辰辰丁倩雯刘宏亮
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap