Content determination method

Abstract

The invention discloses a content determination method, and relates to the technical field of medicine detection, and the content determination method comprises the following steps: S1, taking a decocted extract aqueous solution which contains calycosin-7-glucoside; S2, extracting the aqueous solution by an online solid-phase extraction method to obtain a calycosin-7-glucoside aqueous solution; and S3, measuring the calycosin-7-glucoside aqueous solution by using a high performance liquid chromatography so as to determine the content of the calycosin-7-glucoside. According to the invention, when calycosin-7-glucoside in the decocted extract is measured, on-line solid phase extraction and high performance liquid chromatography are combined; the method can overcome the defect that calycosin-7-glucoside is not easy to separate out due to high viscosity of the decocted extract, is good in repeatability, high in sensitivity and short in analysis time, can be used as a method for determiningthe content of calycosin-7-glucoside in liver and kidney nourishing, and further lays a certain foundation for perfecting the quality standard of the decocted extract and ensuring the medicine quality of the decocted extract.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a content determination method. Background technique [0002] At present, Ganshenzi is a decoction made from wolfberry, astragalus, codonopsis radix, Radix Ophiopogon japonicus, and donkey-hide gelatin. It has the effects of nourishing the liver and improving eyesight, nourishing yin and nourishing the kidney; it is used for deficiency of kidney yin, deficiency of both qi and blood, dizziness and darkness, upset and insomnia, fatigue and weakness of limbs, weakness of waist and legs, etc. According to the compatibility characteristics of the prescriptions, the content of betaine in Lycium barbarum and calycosin glucoside in Astragalus membranaceus was used as the quality control standard for liver and kidney nourishing. The 2015 edition of "Chinese Pharmacopoeia" stipulates that calycocoside glucoside in astragalus can be determined by high performance liquid chromatog...

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Problems solved by technology

The 2015 edition of "Chinese Pharmacopoeia" stipulates that calycocoside glucoside in astragalus can be determined by high performance liquid chromatography, but the semi-fluid preparation made of liver and kidney nourishing medicinal materials after decocting with water, removing residue and concentrating, and adding refined honey has a high viscosity , the sensitivity of the traditional high-performance liquid phase method is low. In order to further improve the quality standards of the decoction and ensure the quality of its medicines, it is necessary to establish a method for the determination of the content of calycosin glucoside in the decoction.

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