A kind of spore-based heavy metal cleaning soap and preparation method thereof
A heavy metal and cleaning soap technology, applied in the direction of chemical instruments and methods, soap detergents and other compounding agents, soap detergent compositions, etc., to achieve increased friction, good cleaning effect, and easy to use in the field
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Embodiment 1
[0032] The preparation of embodiment 1 Bacillus pumilus activated carbon immobilized spore dry powder
[0033] The preparation of bacillus pumilus pumilus gac-immobilized spore dry powder comprises the following steps:
[0034] (1) Acquisition of spores: Take the activated Bacillus pumilus and add it to the sterilized beef extract liquid medium, and culture it with shaking at 32°C and 150rpm for 24 hours. When the cell growth reaches a stable period, raise the culture temperature to 60°C and continue to cultivate After 5h, centrifuge at 11000rpm for 10min to collect the precipitate, resuspend in sterile water and wash 3 times to obtain the spores; activation of Bacillus pumilus: take the freeze-dried powder of the strain and dissolve it in sterile normal saline, take 1 The ring was streaked on the solid medium containing 10% beef extract, and cultivated in a 30°C incubator for 24h to obtain activated Bacillus pumilus;
[0035] (2) Preparation of dry spore powder: sterilize th...
Embodiment 2
[0037] Example 2 Spore-Based Heavy Metal Cleansing Soap
[0038] The heavy metal cleansing soap based on spores comprises the following preparation raw materials and their mass percentages: plant soap base 90%, glycerin 3%, activated carbon immobilized spore dry powder 2% obtained in Example 1, sterile water 5%.
[0039] The preparation method of the heavy metal cleansing soap based on spores comprises the steps of: taking plant soap base, glycerin, activated carbon immobilized spore dry powder and sterile water according to the formula, dissolving completely at 55°C and stirring evenly, then importing mold at 25°C Down solidification molding, obtain the heavy metal cleansing soap based on spore.
Embodiment 3
[0049] The cleaning effect of cleaning soap of embodiment 3 tungsten mine mining plant dust
[0050] The dust in the open-air operation area was sampled in a tungsten mine factory in Jiangxi, and the cleaning effect of heavy metal cleansing soap based on spores was analyzed after the dust polluted the skin. For the content of heavy metals, refer to the relevant methods of "Determination of Copper, Zinc, Lead and Cadmium in Water Quality by Atomic Absorption Spectrophotometry" (GB7475-1987).
[0051] The experimental steps are as follows:
[0052] (1) Dust pollution: take 10×10cm pig back skin as the simulated skin, and evenly apply a layer of dust with a weight of 0.1g on it. Make multiple pieces and leave it for 2 hours to form dust and pollute the skin.
[0053] (2) Soap making: Prepare heavy metal cleansing soap, soap base soap, active carbon soap, unimmobilized spore dry powder soap respectively according to embodiment 2, comparative example 1-3.
[0054] (3) Cleaning o...
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